HS2ST1 ELISA Kit

Catalog No. ABIN5655045

Product Details HS2ST1 ELISA Kit

Target: HS2ST1 (Heparan Sulfate 2-O-Sulfotransferase 1 (HS2ST1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Heparan Sulfate-2-O-Sulfotransferase 1 (HS2ST1). No significant cross-reactivity or interference between Heparan Sulfate-2-O-Sulfotransferase 1 (HS2ST1) and analogues was observed.

Sensitivity: 0.052 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparan Sulfate-2-O-Sulfotransferase 1 (HS2ST1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heparan Sulfate-2-O-Sulfotransferase 1 (HS2ST1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparan Sulfate-2-O-Sulfotransferase 1 (HS2ST1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heparan Sulfate-2-O-Sulfotransferase 1 (HS2ST1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heparan Sulfate-2-O-Sulfotransferase 1 (HS2ST1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heparan Sulfate-2-O-Sulfotransferase 1 (HS2ST1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for HS2ST1

Target: HS2ST1 (Heparan Sulfate 2-O-Sulfotransferase 1 (HS2ST1))

Alternative Name: Heparan Sulfate-2-O-Sulfotransferase 1 (HS2ST1 Products)

Synonyms: HS2ST1 ELISA Kit, hs2st ELISA Kit, dJ604K5.2 ELISA Kit, AW214369 ELISA Kit, Hs2st ELISA Kit, mKIAA0448 ELISA Kit, CHS2ST ELISA Kit, HS2ST ELISA Kit, 2-OST ELISA Kit, 2OST ELISA Kit, HS2st ELISA Kit, hs2st1 ELISA Kit, im:7147474 ELISA Kit, zgc:158230 ELISA Kit, heparan sulfate 2-O-sulfotransferase 1 ELISA Kit, Heparan sulfate 2-O-sulfotransferase 1 ELISA Kit, heparan sulfate 2-O-sulfotransferase 1 L homeolog ELISA Kit, heparan sulfate 2-O-sulfotransferase 1a ELISA Kit, HS2ST1 ELISA Kit, Tsp_05909 ELISA Kit, hs2st1 ELISA Kit, hs2st ELISA Kit, Hs2st1 ELISA Kit, hs2st1.L ELISA Kit, hs2st1a ELISA Kit

Background:

Gene Name: Heparan Sulfate-2-O-Sulfotransferase 1

Gene ID: 9653

UniProt: Q7LGA3

Pathways: Glycosaminoglycan Metabolic Process, Tube Formation, SARS-CoV-2 Protein Interactome