HSP90AA1 ELISA Kit

Catalog No. ABIN5654979

Product Details HSP90AA1 ELISA Kit

Target: HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Heat Shock Protein 90 kDa Alpha A1 (HSP90aA1). No significant cross-reactivity or interference between Heat Shock Protein 90 kDa Alpha A1 (HSP90aA1) and analogues was observed.

Sensitivity: 0.65 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 90 kDa Alpha A1 (HSP90aA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 90 kDa Alpha A1 (HSP90aA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 90 kDa Alpha A1 (HSP90aA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein 90 kDa Alpha A1 (HSP90aA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Protein 90kDa Alpha A1 (HSP90aA1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Protein 90kDa Alpha A1 (HSP90aA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for HSP90AA1

Target: HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))

Alternative Name: Heat Shock Protein 90kDa Alpha A1 (HSP90AA1 Products)

Synonyms: EL52 ELISA Kit, HSP86 ELISA Kit, HSP89A ELISA Kit, HSP90A ELISA Kit, HSP90N ELISA Kit, HSPC1 ELISA Kit, HSPCA ELISA Kit, HSPCAL1 ELISA Kit, HSPCAL4 ELISA Kit, HSPN ELISA Kit, Hsp89 ELISA Kit, Hsp90 ELISA Kit, LAP2 ELISA Kit, Hsp86 ELISA Kit, Hspca ELISA Kit, htpG ELISA Kit, 86kDa ELISA Kit, 89kDa ELISA Kit, AL024080 ELISA Kit, AL024147 ELISA Kit, Hsp86-1 ELISA Kit, hsp4 ELISA Kit, HSP90 ELISA Kit, HSP90AA1 ELISA Kit, fb17b01 ELISA Kit, hsp90 ELISA Kit, hsp90a ELISA Kit, hsp90a.1 ELISA Kit, hsp90alpha ELISA Kit, wu:fb17b01 ELISA Kit, zgc:86652 ELISA Kit, Hsp90alpha ELISA Kit, heat shock protein 90 alpha family class A member 1 ELISA Kit, heat shock protein 90, alpha (cytosolic), class A member 1 ELISA Kit, Heat Shock Protein 90, cytosolic ELISA Kit, heat shock protein 90A ELISA Kit, molecular chaperone ELISA Kit, heat shock protein 90, alpha (cytosolic), class A member 1, tandem duplicate 1 ELISA Kit, heat shock protein HSP 90-alpha ELISA Kit, heat shock protein 90kDa alpha (cytosolic), class A member 1 ELISA Kit, HSP90AA1 ELISA Kit, Hsp90aa1 ELISA Kit, HSP90A ELISA Kit, hsp90A ELISA Kit, hsp90aa1.1 ELISA Kit, LOC108698781 ELISA Kit

Background:

Gene Name: Heat Shock Protein 90 kDa Alpha A1

Gene Aliases: HSP86, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2, Lipopolysaccharide-associated protein 2, Renal carcinoma antigen NY-REN-38

Pathways: M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals