SERPINH1 ELISA Kit

Catalog No. ABIN5654974

Product Details SERPINH1 ELISA Kit

Target: SERPINH1 (Serpin Family H Member 1 (SERPINH1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 125.00 pg/mL - 8000 pg/mL

Minimum Detection Limit: 125.00 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Heat Shock Protein 47 (HSP47). No significant cross-reactivity or interference between Heat Shock Protein 47 (HSP47) and analogues was observed.

Sensitivity: 50 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 47 (HSP47). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 47 (HSP47). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 47 (HSP47), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein 47 (HSP47) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Protein 47 (HSP47) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Protein 47 (HSP47) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SERPINH1

Target: SERPINH1 (Serpin Family H Member 1 (SERPINH1))

Alternative Name: Heat Shock Protein 47 (SERPINH1 Products)

Synonyms: AsTP3 ELISA Kit, CBP1 ELISA Kit, CBP2 ELISA Kit, HSP47 ELISA Kit, OI10 ELISA Kit, PPROM ELISA Kit, RA-A47 ELISA Kit, SERPINH2 ELISA Kit, gp46 ELISA Kit, SERPINH1 ELISA Kit, DKFZp468B197 ELISA Kit, cb216 ELISA Kit, hsp ELISA Kit, hsp47 ELISA Kit, id:ibd5060 ELISA Kit, wu:fc56b02 ELISA Kit, BERF-1 ELISA Kit, Cbp1 ELISA Kit, Cbp2 ELISA Kit, Hsp47 ELISA Kit, J6 ELISA Kit, Serpinh2 ELISA Kit, colligin ELISA Kit, astp3 ELISA Kit, cbp1 ELISA Kit, cbp2 ELISA Kit, pig14 ELISA Kit, pprom ELISA Kit, ra-a47 ELISA Kit, serpinh2 ELISA Kit, serpin family H member 1 ELISA Kit, serpin peptidase inhibitor, clade H (heat shock protein 47), member 1b ELISA Kit, serpin H1 ELISA Kit, selenoprotein K ELISA Kit, serine (or cysteine) peptidase inhibitor, clade H, member 1 ELISA Kit, serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) L homeolog ELISA Kit, SERPINH1 ELISA Kit, serpinh1b ELISA Kit, LOC100009353 ELISA Kit, Selenok ELISA Kit, Serpinh1 ELISA Kit, serpinh1.L ELISA Kit

Target Type: Viral Protein

Background:

Gene Name: Heat Shock Protein 47

Gene Aliases: CBP2, AsTP3, CBP1, PIG14, RA-A47, SERPINH1, SERPINH2, Colligen, Gp46, Serpin Peptidase Inhibitor Clade H Member 1, Heat Shock Protein 47, Collagen Binding Protein 2