HSPA1B ELISA Kit

Catalog No. ABIN5654964

Product Details HSPA1B ELISA Kit

Target: HSPA1B (Heat Shock 70kDa Protein 1B (HSPA1B))

Reactivity: Cow

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Heat Shock 70 kDa Protein 1B (HSPA1B). No significant cross-reactivity or interference between Heat Shock 70 kDa Protein 1B (HSPA1B) and analogues was observed.

Sensitivity: 0.53 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70 kDa Protein 1B (HSPA1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70 kDa Protein 1B (HSPA1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70 kDa Protein 1B (HSPA1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 70 kDa Protein 1B (HSPA1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock 70kDa Protein 1B (HSPA1B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock 70kDa Protein 1B (HSPA1B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for HSPA1B

Target: HSPA1B (Heat Shock 70kDa Protein 1B (HSPA1B))

Alternative Name: Heat Shock 70kDa Protein 1B (HSPA1B Products)

Synonyms: HSP70-1B ELISA Kit, HSP70-2 ELISA Kit, APG-2 ELISA Kit, HS24/P52 ELISA Kit, HSPH2 ELISA Kit, RY ELISA Kit, hsp70 ELISA Kit, hsp70RY ELISA Kit, ARABIDOPSIS THALIANA HEAT SHOCK COGNATE PROTEIN 70-1 ELISA Kit, AT-HSC70-1 ELISA Kit, HEAT SHOCK COGNATE PROTEIN 70 ELISA Kit, HEAT SHOCK PROTEIN 70-1 ELISA Kit, HSC70 ELISA Kit, HSP70-1 ELISA Kit, T22P11.90 ELISA Kit, T22P11_90 ELISA Kit, heat shock cognate protein 70-1 ELISA Kit, Hsp70 ELISA Kit, Hsp70-1 ELISA Kit, Hsp70.1 ELISA Kit, hsp68 ELISA Kit, HSPA1A ELISA Kit, HSPA1B ELISA Kit, HSPA2 ELISA Kit, HSP70.2 ELISA Kit, Hsp70-2 ELISA Kit, Hsp72 ELISA Kit, Hspa1 ELISA Kit, Hspa1a ELISA Kit, Hspa2 ELISA Kit, heat shock protein family A (Hsp70) member 1B ELISA Kit, heat shock protein family A (Hsp70) member 4 ELISA Kit, heat shock cognate protein 70-1 ELISA Kit, heat shock protein 1B ELISA Kit, heat shock protein 70.2 ELISA Kit, heat shock 70kDa protein 1A ELISA Kit, heat shock 70kD protein 1B (mapped) ELISA Kit, HSPA1B ELISA Kit, HSPA4 ELISA Kit, HSC70-1 ELISA Kit, Hspa1b ELISA Kit, HSP70.2 ELISA Kit, HSPA1A ELISA Kit

Background:

Gene Name: Heat Shock 70 kDa Protein 1B

Gene Aliases: HSP70-2, HSP-A1B, Heat Shock Protein 72

Gene ID: 282254

UniProt: Q27965