H2AFX ELISA Kit

Catalog No. ABIN5654929

Product Details H2AFX ELISA Kit

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of H2A Histone Family, Member X (H2AFX). No significant cross-reactivity or interference between H2A Histone Family, Member X (H2AFX) and analogues was observed.

Sensitivity: 0.059 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to H2A Histone Family, Member X (H2AFX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to H2A Histone Family, Member X (H2AFX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain H2A Histone Family, Member X (H2AFX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of H2A Histone Family, Member X (H2AFX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level H2A Histone Family, Member X (H2AFX) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level H2A Histone Family, Member X (H2AFX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Alternative Name: H2A Histone Family, Member X (H2AFX Products)

Synonyms: H2A.X ELISA Kit, H2A/X ELISA Kit, H2AX ELISA Kit, AW228881 ELISA Kit, H2ax ELISA Kit, Hist5-2ax ELISA Kit, gammaH2ax ELISA Kit, zgc:56329 ELISA Kit, h2a.x ELISA Kit, h2a/x ELISA Kit, h2ax ELISA Kit, RGD1566119 ELISA Kit, h2a ELISA Kit, h2afx ELISA Kit, H2A histone family member X ELISA Kit, H2A histone family, member X ELISA Kit, H2A histone family member X L homeolog ELISA Kit, histone cluster 1, H2ah ELISA Kit, histone cluster 2, H2ab S homeolog ELISA Kit, histone H2AX ELISA Kit, H2AFX ELISA Kit, H2afx ELISA Kit, h2afx ELISA Kit, h2afx.L ELISA Kit, HIST1H2AH ELISA Kit, hist2h2ab.S ELISA Kit, LOC100522201 ELISA Kit, LOC100720536 ELISA Kit

Background:

Gene Name: H2A Histone Family, Member X

Gene Aliases: H2AX

Gene ID: 3014

UniProt: P16104

Pathways: Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus