H2AFV ELISA Kit

Catalog No. ABIN5654928

Product Details H2AFV ELISA Kit

Target: H2AFV (H2A Histone Family, Member V (H2AFV))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of H2A Histone Family, Member V (H2AFV). No significant cross-reactivity or interference between H2A Histone Family, Member V (H2AFV) and analogues was observed.

Sensitivity: 0.59 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to H2A Histone Family, Member V (H2AFV). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to H2A Histone Family, Member V (H2AFV). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain H2A Histone Family, Member V (H2AFV), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of H2A Histone Family, Member V (H2AFV) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level H2A Histone Family, Member V (H2AFV) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level H2A Histone Family, Member V (H2AFV) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for H2AFV

Target: H2AFV (H2A Histone Family, Member V (H2AFV))

Alternative Name: H2A Histone Family, Member V (H2AFV Products)

Synonyms: H2A.Z-2 ELISA Kit, H2afv ELISA Kit, H2AV ELISA Kit, h2av ELISA Kit, C530002L11Rik ELISA Kit, H2a.z-2 ELISA Kit, H2av ELISA Kit, H2A.F/Z ELISA Kit, h2afv ELISA Kit, h2afz ELISA Kit, H2A histone family, member V ELISA Kit, H2A histone family member V ELISA Kit, H2A histone family member V S homeolog ELISA Kit, H2A histone family, member Va ELISA Kit, H2afv ELISA Kit, LOC100226437 ELISA Kit, H2AFV ELISA Kit, h2afv.S ELISA Kit, h2afva ELISA Kit

Background:

Gene Name: H2A Histone Family, Member V

Gene Aliases: H2AV

Gene ID: 94239

UniProt: Q71UI9