GLO1 ELISA Kit

Catalog No. ABIN5654775

Product Details GLO1 ELISA Kit

Target: GLO1 (Glyoxalase I (GLO1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Glyoxalase I (GLO1). No significant cross-reactivity or interference between Glyoxalase I (GLO1) and analogues was observed.

Sensitivity: 0.59 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glyoxalase I (GLO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glyoxalase I (GLO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glyoxalase I (GLO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glyoxalase I (GLO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glyoxalase I (GLO1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glyoxalase I (GLO1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for GLO1

Target: GLO1 (Glyoxalase I (GLO1))

Alternative Name: Glyoxalase I (GLO1 Products)

Synonyms: GLOD1 ELISA Kit, GLYI ELISA Kit, 0610009E22Rik ELISA Kit, 1110008E19Rik ELISA Kit, 2510049H23Rik ELISA Kit, AW550643 ELISA Kit, GLY1 ELISA Kit, Glo-1 ELISA Kit, Glo-1r ELISA Kit, Glo-1s ELISA Kit, Glo1-r ELISA Kit, Glo1-s ELISA Kit, Qglo ELISA Kit, cb554 ELISA Kit, zgc:66035 ELISA Kit, wu:fb82g09 ELISA Kit, glod1 ELISA Kit, glyi ELISA Kit, glo1 ELISA Kit, GLO1 ELISA Kit, ATGLX1 ELISA Kit, F12F1.32 ELISA Kit, F12F1_32 ELISA Kit, GLYOXALASE I ELISA Kit, glyoxalase I homolog ELISA Kit, trypanothione-dependent glyoxalase I ELISA Kit, glyoxalase I ELISA Kit, glyoxalase 1 ELISA Kit, glyoxalase 1 L homeolog ELISA Kit, glyoxalase 1 S homeolog ELISA Kit, lactoylglutathione lyase ELISA Kit, glyoxalase/bleomycin resistance protein/dioxygenase superfamily protein ELISA Kit, GLO1 ELISA Kit, Glo1 ELISA Kit, glo1 ELISA Kit, glo1.L ELISA Kit, glo1.S ELISA Kit, GST3 ELISA Kit, GLX1 ELISA Kit, STY1687 ELISA Kit, Bcen_2094 ELISA Kit, gloA ELISA Kit

Background:

Gene Name: Glyoxalase I

Gene Aliases: GLY-I, GLOD1, Glyoxalase Domain Containing 1, Lactoylglutathione lyase, Aldoketomutase, Ketone-aldehyde mutase, Methylglyoxalase, S-D-lactoylglutathione methylglyoxal lyase