Glutathione Synthetase ELISA Kit

Catalog No. ABIN5654725

Product Details Glutathione Synthetase ELISA Kit

Target: Glutathione Synthetase (GSS)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Glutathione Synthetase (GSS). No significant cross-reactivity or interference between Glutathione Synthetase (GSS) and analogues was observed.

Sensitivity: 0.054 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Synthetase (GSS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione Synthetase (GSS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione Synthetase (GSS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione Synthetase (GSS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione Synthetase (GSS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione Synthetase (GSS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Glutathione Synthetase

Target: Glutathione Synthetase (GSS)

Alternative Name: Glutathione Synthetase (GSS Products)

Synonyms: PRNP ELISA Kit, 6835 ELISA Kit, CG32497 ELISA Kit, CG33065 ELISA Kit, CG6835 ELISA Kit, Dmel\\CG6835 ELISA Kit, F21A20.90 ELISA Kit, F21A20_90 ELISA Kit, GLUTATHIONE SYNTHETASE ELISA Kit, GSHB ELISA Kit, glutathione synthetase 2 ELISA Kit, GSHS ELISA Kit, AI314904 ELISA Kit, GS-A/GS-B ELISA Kit, gssls-a ELISA Kit, glutathione synthetase ELISA Kit, Glutathione Synthetase ELISA Kit, glutathione synthetase 2 ELISA Kit, glutathione synthetase L homeolog ELISA Kit, GSS ELISA Kit, GSH2 ELISA Kit, Tola_1250 ELISA Kit, PC1_3711 ELISA Kit, Dd586_3633 ELISA Kit, Kvar_0701 ELISA Kit, Alvin_0197 ELISA Kit, Nhal_0215 ELISA Kit, BC1002_0254 ELISA Kit, Cseg_4017 ELISA Kit, Snov_3844 ELISA Kit, Aazo_1904 ELISA Kit, M301_2613 ELISA Kit, Bresu_0515 ELISA Kit, Isop_1460 ELISA Kit, Marme_4020 ELISA Kit, Thicy_0325 ELISA Kit, Sinme_3668 ELISA Kit, Mesop_0757 ELISA Kit, Gss ELISA Kit, gss.L ELISA Kit

Background:

Gene Name: Glutathione Synthetase

Gene Aliases: Glutathione synthase

Gene ID: 14854

UniProt: P51855

Pathways: Warburg Effect