Glutaredoxin 1 ELISA Kit

Catalog No. ABIN5654687

Product Details Glutaredoxin 1 ELISA Kit

Target: Glutaredoxin 1 (GRX1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Glutaredoxin (GLRX). No significant cross-reactivity or interference between Glutaredoxin (GLRX) and analogues was observed.

Sensitivity: 28.9 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutaredoxin (GLRX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutaredoxin (GLRX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutaredoxin (GLRX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutaredoxin (GLRX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutaredoxin (GLRX) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutaredoxin (GLRX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Glutaredoxin 1

Target: Glutaredoxin 1 (GRX1)

Alternative Name: Glutaredoxin (GRX1 Products)

Synonyms: Glrx1 ELISA Kit, Grx ELISA Kit, grx ELISA Kit, wu:fc38f02 ELISA Kit, zgc:103707 ELISA Kit, GLRXL ELISA Kit, Grx1 ELISA Kit, TTase ELISA Kit, C86710 ELISA Kit, D13Wsu156e ELISA Kit, grx1 ELISA Kit, GLRX ELISA Kit, GRX ELISA Kit, GRX1 ELISA Kit, GLRX1 ELISA Kit, TTF ELISA Kit, glrx ELISA Kit, glutaredoxin ELISA Kit, glutaredoxin (thioltransferase) ELISA Kit, glutaredoxin Grx1 ELISA Kit, NrdH-redoxin ELISA Kit, Uncharacterized monothiol glutaredoxin F10D7.3 ELISA Kit, glutaredoxin-1 (Grx1) ELISA Kit, temporal expression: late ELISA Kit, glutaredoxin L homeolog ELISA Kit, Glrx ELISA Kit, glrx ELISA Kit, GLRX ELISA Kit, grx1 ELISA Kit, AF_RS07750 ELISA Kit, F10D7.3 ELISA Kit, AFUA_1G06100 ELISA Kit, NT01EI_2528 ELISA Kit, O2L ELISA Kit, glrx.L ELISA Kit

Target Type: Viral Protein

Background:

Gene Name: Glutaredoxin

Gene Aliases: GRX, GRX1, Thioltransferase

Gene ID: 2745

UniProt: P35754

Pathways: Cell RedoxHomeostasis