ENPEP ELISA Kit

Catalog No. ABIN5654686

Product Details ENPEP ELISA Kit

Target: ENPEP (Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Glutamyl Aminopeptidase (GluAP). No significant cross-reactivity or interference between Glutamyl Aminopeptidase (GluAP) and analogues was observed.

Sensitivity: 0.062 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamyl Aminopeptidase (GluAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamyl Aminopeptidase (GluAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamyl Aminopeptidase (GluAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamyl Aminopeptidase (GluAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamyl Aminopeptidase (GluAP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamyl Aminopeptidase (GluAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ENPEP

Target: ENPEP (Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP))

Alternative Name: Glutamyl Aminopeptidase (ENPEP Products)

Synonyms: im:7152184 ELISA Kit, si:ch211-146m5.2 ELISA Kit, 6030431M22Rik ELISA Kit, APA ELISA Kit, Bp-1/6C3 ELISA Kit, Ly-51 ELISA Kit, Ly51 ELISA Kit, CD249 ELISA Kit, gp160 ELISA Kit, glutamyl aminopeptidase ELISA Kit, ENPEP ELISA Kit, enpep ELISA Kit, ECU01_1470 ELISA Kit, pepA ELISA Kit, LOC5568916 ELISA Kit, CpipJ_CPIJ011103 ELISA Kit, Eint_011390 ELISA Kit, Halhy_0222 ELISA Kit, Enpep ELISA Kit

Background:

Gene Name: Glutamyl Aminopeptidase

Gene Aliases: CD249, ENPEP, gp160, ATA, EAP, AP-A, Aminopeptidase A

Gene ID: 13809

UniProt: P16406

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones