QPCT ELISA Kit

Catalog No. ABIN5654685

Product Details QPCT ELISA Kit

Target: QPCT (Glutaminyl-Peptide Cyclotransferase (QPCT))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Glutaminyl Peptide Cyclotransferase (QPCT). No significant cross-reactivity or interference between Glutaminyl Peptide Cyclotransferase (QPCT) and analogues was observed.

Sensitivity: 0.065 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutaminyl Peptide Cyclotransferase (QPCT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutaminyl Peptide Cyclotransferase (QPCT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutaminyl Peptide Cyclotransferase (QPCT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutaminyl Peptide Cyclotransferase (QPCT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutaminyl Peptide Cyclotransferase (QPCT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutaminyl Peptide Cyclotransferase (QPCT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for QPCT

Target: QPCT (Glutaminyl-Peptide Cyclotransferase (QPCT))

Alternative Name: Glutaminyl Peptide Cyclotransferase (QPCT Products)

Synonyms: GCT ELISA Kit, QC ELISA Kit, sQC ELISA Kit, QPCT ELISA Kit, 5730422A13Rik ELISA Kit, Qpctl1 ELISA Kit, RGD1562284 ELISA Kit, qpct ELISA Kit, glutaminyl-peptide cyclotransferase ELISA Kit, glutaminyl-peptide cyclotransferase (glutaminyl cyclase) ELISA Kit, glutaminyl-peptide cyclotransferase L homeolog ELISA Kit, QPCT ELISA Kit, Qpct ELISA Kit, M6_Spy0443 ELISA Kit, M5005_Spy_0416 ELISA Kit, M28_Spy0404 ELISA Kit, CJA_2408 ELISA Kit, CCNA_02733 ELISA Kit, Tsp_03182 ELISA Kit, qpct.L ELISA Kit

Background:

Gene Name: Glutaminyl Peptide Cyclotransferase

Gene Aliases: GCT, QC, EC, Glutaminyl Cyclase, Glutaminyl-tRNA cyclotransferase

Gene ID: 25797

UniProt: Q16769