GAD65 ELISA Kit

Catalog No. ABIN5654650

Product Details GAD65 ELISA Kit

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.78 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Glutamate Decarboxylase 2, Acid (GAD2). No significant cross-reactivity or interference between Glutamate Decarboxylase 2, Acid (GAD2) and analogues was observed.

Sensitivity: 0.27 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Decarboxylase 2, Acid (GAD2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Decarboxylase 2, Acid (GAD2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Decarboxylase 2, Acid (GAD2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Decarboxylase 2, Acid (GAD2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Decarboxylase 2, Acid (GAD2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Decarboxylase 2, Acid (GAD2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for GAD65

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Alternative Name: Glutamate Decarboxylase 2, Acid (GAD2 Products)

Synonyms: GAD65 ELISA Kit, gad65 ELISA Kit, F12P19.12 ELISA Kit, F12P19_12 ELISA Kit, GLUTAMATE DECARBOXYLASE 2 ELISA Kit, glutamate decarboxylase 2 ELISA Kit, zgc:112198 ELISA Kit, 6330404F12Rik ELISA Kit, GAD(65) ELISA Kit, Gad-2 ELISA Kit, glutamate decarboxylase 2 ELISA Kit, glutamate decarboxylase 1 ELISA Kit, glutamic acid decarboxylase 2 ELISA Kit, GAD2 ELISA Kit, PTRG_06148 ELISA Kit, LOC9324534 ELISA Kit, Gad2 ELISA Kit, GAD1 ELISA Kit, gad2 ELISA Kit

Background:

Gene Name: Glutamate Decarboxylase 2, Acid

Gene Aliases: GAD65, Pancreatic Islets And Brain,65 kDa, 65 kDa glutamic acid decarboxylase, Glutamate decarboxylase 65 kDa isoform

UniProt: Q05329