GLUT1 ELISA Kit
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- Target See all GLUT1 (SLC2A1) ELISA Kits
- GLUT1 (SLC2A1) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 1 (SLC2A1))
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Reactivity
- Rat
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 1.25 ng/mL - 80 ng/mL
- Minimum Detection Limit
- 1.25 ng/mL
- Application
- ELISA
- Sample Type
- Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Glucose Transporter 1 (GLUT1). No significant cross-reactivity or interference between Glucose Transporter 1 (GLUT1) and analogues was observed.
- Sensitivity
- 0.48 ng/mL
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 3 h
- Plate
- Pre-coated
- Protocol
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose Transporter 1 (GLUT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose Transporter 1 (GLUT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose Transporter 1 (GLUT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucose Transporter 1 (GLUT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucose Transporter 1 (GLUT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucose Transporter 1 (GLUT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all GLUT1 (SLC2A1) ELISA Kits
- GLUT1 (SLC2A1) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 1 (SLC2A1))
- Alternative Name
- Glucose Transporter 1 (SLC2A1 Products)
- Synonyms
- DYT17 ELISA Kit, DYT18 ELISA Kit, DYT9 ELISA Kit, EIG12 ELISA Kit, GLUT ELISA Kit, GLUT1 ELISA Kit, GLUT1DS ELISA Kit, HTLVR ELISA Kit, PED ELISA Kit, Glut-1 ELISA Kit, Glut1 ELISA Kit, GLUTB ELISA Kit, GTG1 ELISA Kit, Gtg3 ELISA Kit, RATGTG1 ELISA Kit, SLC2A1 ELISA Kit, GLT1 ELISA Kit, GLUCOSE TRANSPORTER 1 ELISA Kit, T21H19.70 ELISA Kit, T21H19_70 ELISA Kit, plastidic GLC translocator ELISA Kit, glut1 ELISA Kit, ped ELISA Kit, slc2a1 ELISA Kit, slc2a14 ELISA Kit, solute carrier family 2 member 1 ELISA Kit, solute carrier family 2 (facilitated glucose transporter), member 1 ELISA Kit, plastidic GLC translocator ELISA Kit, solute carrier family 2 (facilitated glucose transporter), member 1 L homeolog ELISA Kit, SLC2A1 ELISA Kit, Slc2a1 ELISA Kit, PGLCT ELISA Kit, slc2a1.L ELISA Kit
- Background
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Gene Name: Glucose Transporter 1
Gene Aliases: SLC2A1, SLC2-A1, GLUT, Solute Carrier Family 2 Member 1,Facilitated Glucose Transporter, Glucose transporter type 1, erythrocyte/brain, HepG2 glucose transporter
- Pathways
- Sensory Perception of Sound, Dicarboxylic Acid Transport, Warburg Effect
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