GPER ELISA Kit

Catalog No. ABIN5654455

Product Details GPER ELISA Kit

Target: GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of G Protein Coupled Estrogen Receptor 1 (GPER). No significant cross-reactivity or interference between G Protein Coupled Estrogen Receptor 1 (GPER) and analogues was observed.

Sensitivity: 0.054 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Coupled Estrogen Receptor 1 (GPER). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to G Protein Coupled Estrogen Receptor 1 (GPER). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Coupled Estrogen Receptor 1 (GPER), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of G Protein Coupled Estrogen Receptor 1 (GPER) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level G Protein Coupled Estrogen Receptor 1 (GPER) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level G Protein Coupled Estrogen Receptor 1 (GPER) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for GPER

Target: GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))

Alternative Name: G Protein Coupled Estrogen Receptor 1 (GPER Products)

Synonyms: CEPR ELISA Kit, CMKRL2 ELISA Kit, DRY12 ELISA Kit, FEG-1 ELISA Kit, GPCR-Br ELISA Kit, GPER ELISA Kit, GPR30 ELISA Kit, LERGU ELISA Kit, LERGU2 ELISA Kit, LyGPR ELISA Kit, 6330420K13Rik ELISA Kit, Ceprl ELISA Kit, Gper ELISA Kit, Gpr30 ELISA Kit, GPR41 ELISA Kit, GPCR30 ELISA Kit, G protein-coupled estrogen receptor 1 ELISA Kit, GPER1 ELISA Kit, GPER ELISA Kit, Gper1 ELISA Kit

Background:

Gene Name: G Protein Coupled Estrogen Receptor 1

Gene Aliases: GPR30, CMKRL2, DRY12, FEG-1, GPCR-Br, LERGU, LyGPR, Membrane estrogen receptor, Chemoattractant receptor-like 2, Flow-induced endothelial G-protein coupled receptor 1

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process