FAH ELISA Kit

Catalog No. ABIN5654443

Product Details FAH ELISA Kit

Target: FAH (Fumarylacetoacetate Hydrolase (Fumarylacetoacetase) (FAH))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Fumarylacetoacetate Hydrolase (FAH). No significant cross-reactivity or interference between Fumarylacetoacetate Hydrolase (FAH) and analogues was observed.

Sensitivity: 0.058 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fumarylacetoacetate Hydrolase (FAH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fumarylacetoacetate Hydrolase (FAH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fumarylacetoacetate Hydrolase (FAH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fumarylacetoacetate Hydrolase (FAH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fumarylacetoacetate Hydrolase (FAH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fumarylacetoacetate Hydrolase (FAH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for FAH

Target: FAH (Fumarylacetoacetate Hydrolase (Fumarylacetoacetase) (FAH))

Alternative Name: Fumarylacetoacetate Hydrolase (FAH Products)

Synonyms: CG14993 ELISA Kit, Dmel\\CG14993 ELISA Kit, dfaa ELISA Kit, l(3)64Al ELISA Kit, l(3)SH11 ELISA Kit, PSPTO3550 ELISA Kit, fb59b12 ELISA Kit, wu:fb59b12 ELISA Kit, zgc:55316 ELISA Kit, FAA ELISA Kit, Fumarylacetoacetase ELISA Kit, FumarylAcetoacetate Hydrolase ELISA Kit, fumarylacetoacetase ELISA Kit, fumarylacetoacetate hydrolase ELISA Kit, fumarylacetoacetate hydrolase (fumarylacetoacetase) ELISA Kit, fumarylacetoacetate hydrolase (fumarylacetoacetase) L homeolog ELISA Kit, Faa ELISA Kit, fah-1 ELISA Kit, fahA ELISA Kit, hmgB ELISA Kit, Ndas_1870 ELISA Kit, Lbys_3503 ELISA Kit, Riean_0663 ELISA Kit, Ftrac_0820 ELISA Kit, Celal_3675 ELISA Kit, Celly_2655 ELISA Kit, Weevi_0208 ELISA Kit, Fluta_2435 ELISA Kit, Halhy_2019 ELISA Kit, Lacal_2931 ELISA Kit, FsymDg_1892 ELISA Kit, Fah ELISA Kit, fah ELISA Kit, fah.L ELISA Kit, FAH ELISA Kit

Background:

Gene Name: Fumarylacetoacetate Hydrolase

Gene Aliases: FAA, Fumarylacetoacetase, Tyrosinemia 1, Beta-diketonase

Gene ID: 2184

UniProt: P16930