FUCA1 ELISA Kit
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- Target See all FUCA1 ELISA Kits
- FUCA1 (Fucosidase, alpha-L- 1, Tissue (FUCA1))
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Reactivity
- Rat
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.312 ng/mL - 20 ng/mL
- Minimum Detection Limit
- 0.312 ng/mL
- Application
- ELISA
- Sample Type
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Fucosidase Alpha L1, Tissue (FUCa1). No significant cross-reactivity or interference between Fucosidase Alpha L1, Tissue (FUCa1) and analogues was observed.
- Sensitivity
- 0.115 ng/mL
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 3 h
- Plate
- Pre-coated
- Protocol
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fucosidase Alpha L1, Tissue (FUCa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fucosidase Alpha L1, Tissue (FUCa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fucosidase Alpha L1, Tissue (FUCa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fucosidase Alpha L1, Tissue (FUCa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fucosidase Alpha L1, Tissue (FUCa1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fucosidase Alpha L1, Tissue (FUCa1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all FUCA1 ELISA Kits
- FUCA1 (Fucosidase, alpha-L- 1, Tissue (FUCA1))
- Alternative Name
- Fucosidase Alpha L1, Tissue (FUCA1 Products)
- Synonyms
- FUCA ELISA Kit, FUCA1 ELISA Kit, 0610006A03Rik ELISA Kit, 9530055J05Rik ELISA Kit, Afuc ELISA Kit, Fuca ELISA Kit, fuca ELISA Kit, fuca1 ELISA Kit, ATFUC1 ELISA Kit, F24D13.11 ELISA Kit, F24D13_11 ELISA Kit, alpha-L-fucosidase 1 ELISA Kit, wu:fb02a09 ELISA Kit, zgc:101116 ELISA Kit, alpha-L-fucosidase 1 ELISA Kit, fucosidase, alpha-L- 1, tissue ELISA Kit, alpha-L-fucosidase 1 L homeolog ELISA Kit, alpha-L-fucosidase 1, tandem duplicate 2 ELISA Kit, FUCA1 ELISA Kit, Fuca1 ELISA Kit, fuca1.L ELISA Kit, fuca1 ELISA Kit, FUC1 ELISA Kit, LOC9317020 ELISA Kit, LOC100285202 ELISA Kit, fuca1.2 ELISA Kit
- Background
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Gene Name: Fucosidase Alpha L1, Tissue
Gene Aliases: A-FU, aFU, Alpha-L-Fucosidase, Alpha-L-fucoside fucohydrolase 1
- Gene ID
- 24375
- UniProt
- P17164
- Pathways
- Glycosaminoglycan Metabolic Process
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