FMR1 ELISA Kit

Catalog No. ABIN5654421

Product Details FMR1 ELISA Kit

Target: FMR1 (Fragile X Mental Retardation 1 (FMR1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Fragile X Mental Retardation 1 (FMR1). No significant cross-reactivity or interference between Fragile X Mental Retardation 1 (FMR1) and analogues was observed.

Sensitivity: 0.113 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fragile X Mental Retardation 1 (FMR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fragile X Mental Retardation 1 (FMR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fragile X Mental Retardation 1 (FMR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fragile X Mental Retardation 1 (FMR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fragile X Mental Retardation 1 (FMR1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fragile X Mental Retardation 1 (FMR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for FMR1

Target: FMR1 (Fragile X Mental Retardation 1 (FMR1))

Alternative Name: Fragile X Mental Retardation 1 (FMR1 Products)

Synonyms: AT24755 ELISA Kit, BcDNA:GM08679 ELISA Kit, CG6203 ELISA Kit, Dmel\\CG6203 ELISA Kit, EP(3)3517 ELISA Kit, FMR ELISA Kit, FMR1 ELISA Kit, FMRP ELISA Kit, FMRp ELISA Kit, FXR ELISA Kit, Fmrp ELISA Kit, cg6203 ELISA Kit, dFMR ELISA Kit, dFMR1 ELISA Kit, dFMRP ELISA Kit, dFXR ELISA Kit, dFXR1 ELISA Kit, dFXRP ELISA Kit, dFmr1 ELISA Kit, dFmrp ELISA Kit, dfmr ELISA Kit, dfmr1 ELISA Kit, dfxr ELISA Kit, dfxr1 ELISA Kit, dmfr1 ELISA Kit, fmr ELISA Kit, fmr1 ELISA Kit, FRAXA ELISA Kit, POF ELISA Kit, POF1 ELISA Kit, zFMR1 ELISA Kit, Fmr-1 ELISA Kit, CG6203 gene product from transcript CG6203-RC ELISA Kit, fragile X mental retardation 1 ELISA Kit, fragile X mental retardation syndrome 1 ELISA Kit, Fmr1 ELISA Kit, FMR1 ELISA Kit, fmr1 ELISA Kit

Background:

Gene Name: Fragile X Mental Retardation 1

Gene Aliases: FMRP, FRAXA

Gene ID: 2332

UniProt: Q06787

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development