FHL1 ELISA Kit

Catalog No. ABIN5654420

Product Details FHL1 ELISA Kit

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Four And A Half LIM Domains Protein 1 (FHL1). No significant cross-reactivity or interference between Four And A Half LIM Domains Protein 1 (FHL1) and analogues was observed.

Sensitivity: 0.058 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Four And A Half LIM Domains Protein 1 (FHL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Four And A Half LIM Domains Protein 1 (FHL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Four And A Half LIM Domains Protein 1 (FHL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Four And A Half LIM Domains Protein 1 (FHL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Four And A Half LIM Domains Protein 1 (FHL1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Four And A Half LIM Domains Protein 1 (FHL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for FHL1

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Alternative Name: Four And A Half LIM Domains Protein 1 (FHL1 Products)

Synonyms: fhl1 ELISA Kit, MGC89165 ELISA Kit, FHL1 ELISA Kit, DKFZp468O1927 ELISA Kit, fhl ELISA Kit, fhla ELISA Kit, zgc:92025 ELISA Kit, FHL-1 ELISA Kit, FHL1A ELISA Kit, FHL1B ELISA Kit, FLH1A ELISA Kit, KYOT ELISA Kit, SLIM ELISA Kit, SLIM-1 ELISA Kit, SLIM1 ELISA Kit, SLIMMER ELISA Kit, XMPMA ELISA Kit, KyoT ELISA Kit, Fhl1 ELISA Kit, four and a half LIM domains 1 ELISA Kit, four and a half LIM domains 1a ELISA Kit, Four and a half LIM domains protein 1 ELISA Kit, four and a half LIM domains 1 S homeolog ELISA Kit, fhl1 ELISA Kit, FHL1 ELISA Kit, fhl1a ELISA Kit, fhl1.S ELISA Kit, Fhl1 ELISA Kit

Background:

Gene Name: Four And A Half LIM Domains Protein 1

Gene Aliases: FHL1B, KYO-T, SLIM1, XMPMA, FLH1A, LIM Protein SLIMMER, Skeletal muscle LIM-protein 1