FKBP4 ELISA Kit

Catalog No. ABIN5654360

Product Details FKBP4 ELISA Kit

Target: FKBP4 (FK506 Binding Protein 4, 59kDa (FKBP4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of FK506 Binding Protein 4 (FKBP4). No significant cross-reactivity or interference between FK506 Binding Protein 4 (FKBP4) and analogues was observed.

Sensitivity: 0.059 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FK506 Binding Protein 4 (FKBP4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FK506 Binding Protein 4 (FKBP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FK506 Binding Protein 4 (FKBP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FK506 Binding Protein 4 (FKBP4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FK506 Binding Protein 4 (FKBP4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FK506 Binding Protein 4 (FKBP4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for FKBP4

Target: FKBP4 (FK506 Binding Protein 4, 59kDa (FKBP4))

Alternative Name: FK506 Binding Protein 4 (FKBP4 Products)

Synonyms: HBI ELISA Kit, p52 ELISA Kit, hsp56 ELISA Kit, PPIase ELISA Kit, fkbp52 ELISA Kit, fkbp59 ELISA Kit, MGC81078 ELISA Kit, wu:fb34f09 ELISA Kit, 59kDa ELISA Kit, AL022792 ELISA Kit, AW208983 ELISA Kit, FKBP-4 ELISA Kit, FKBP-52 ELISA Kit, FKBP52 ELISA Kit, FKPB52 ELISA Kit, p59 ELISA Kit, FKBP51 ELISA Kit, FKBP59 ELISA Kit, Hsp56 ELISA Kit, P59 ELISA Kit, FK506 binding protein 4 ELISA Kit, FK506 binding protein 4 S homeolog ELISA Kit, FKBP4 ELISA Kit, fkbp4.S ELISA Kit, fkbp4 ELISA Kit, Fkbp4 ELISA Kit

Background:

Gene Name: FK506 Binding Protein 4

Gene Aliases: FKBP52, FKBP59, HBI, Hsp56, p52, Rotamase, 51 kDa FK506-binding protein, 59 kDa immunophilin, HSP-binding immunophilin, Peptidyl-prolyl cis-trans isomerase FKBP4

Gene ID: 2288

UniProt: Q02790

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway