FKBP1A ELISA Kit

Catalog No. ABIN5654357

Product Details FKBP1A ELISA Kit

Target: FKBP1A (FK506 Binding Protein 1A, 12kDa (FKBP1A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of FK506 Binding Protein 1A (FKBP1A). No significant cross-reactivity or interference between FK506 Binding Protein 1A (FKBP1A) and analogues was observed.

Sensitivity: 11.8 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FK506 Binding Protein 1A (FKBP1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FK506 Binding Protein 1A (FKBP1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FK506 Binding Protein 1A (FKBP1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FK506 Binding Protein 1A (FKBP1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FK506 Binding Protein 1A (FKBP1A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FK506 Binding Protein 1A (FKBP1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for FKBP1A

Target: FKBP1A (FK506 Binding Protein 1A, 12kDa (FKBP1A))

Alternative Name: FK506 Binding Protein 1A (FKBP1A Products)

Synonyms: 12kDa ELISA Kit, FKBP12 ELISA Kit, FKBP12-T1 ELISA Kit, FKBP12-T2 ELISA Kit, Fkbp ELISA Kit, Fkbp1 ELISA Kit, mFKBP1 ELISA Kit, mFKBP12 ELISA Kit, FKBP-12 ELISA Kit, FKBP-1A ELISA Kit, FKBP1 ELISA Kit, Rotamase ELISA Kit, Fkbp12 ELISA Kit, ARABIDOPSIS THALIANA FK506-BINDING PROTEIN 12 ELISA Kit, ATFKBP12 ELISA Kit, FK506-binding protein 12 ELISA Kit, IMMUNOPHILIN ELISA Kit, MSJ1.19 ELISA Kit, MSJ1_19 ELISA Kit, PKC12 ELISA Kit, PKCI2 ELISA Kit, PPIASE ELISA Kit, FKBP1B ELISA Kit, Fkbp2 ELISA Kit, fj02g10 ELISA Kit, zgc:73373 ELISA Kit, wu:fj02g10 ELISA Kit, FKBP1A ELISA Kit, Bmfkbp13 ELISA Kit, zgc:103752 ELISA Kit, FK506 binding protein 1a ELISA Kit, FK506 binding protein 1A ELISA Kit, FK506-binding protein ELISA Kit, FK506-binding protein 12 ELISA Kit, FK506 binding protein 1Aa ELISA Kit, FK506 binding protein 1C ELISA Kit, FK506-binding protein 59 ELISA Kit, FK506 binding protein like ELISA Kit, FK506 binding protein 1A L homeolog ELISA Kit, FK506 binding protein 1Ab ELISA Kit, Fkbp1a ELISA Kit, FKBP1A ELISA Kit, Fkbp12 ELISA Kit, FKBP12 ELISA Kit, fkbp1aa ELISA Kit, FKBP1C ELISA Kit, EHI_180160 ELISA Kit, LOC5573443 ELISA Kit, Fkbp13 ELISA Kit, FKBPL ELISA Kit, fkbp1a.L ELISA Kit, fkbp1ab ELISA Kit

Background:

Gene Name: FK506 Binding Protein 1A

Gene Aliases: FKBP-12, FKBP1, FKBP12C, PKC12, PKCI2, Calstabin-1, Rotamase, 12 kDa FK506-binding protein, Peptidyl-prolyl cis-trans isomerase FKBP1A, Immunophilin FKBP12

Gene ID: 2280

UniProt: P62942

Pathways: Negative Regulation of Transporter Activity, Methionine Biosynthetic Process