FBLN4 ELISA Kit

Catalog No. ABIN5654338

Product Details FBLN4 ELISA Kit

Target: FBLN4 (Fibulin 4 (FBLN4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.12 ng/mL - 200 ng/mL

Minimum Detection Limit: 3.12 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Fibulin 4 (FBLN4). No significant cross-reactivity or interference between Fibulin 4 (FBLN4) and analogues was observed.

Sensitivity: 1.32 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibulin 4 (FBLN4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibulin 4 (FBLN4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibulin 4 (FBLN4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibulin 4 (FBLN4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibulin 4 (FBLN4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibulin 4 (FBLN4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for FBLN4

Target: FBLN4 (Fibulin 4 (FBLN4))

Alternative Name: Fibulin 4 (FBLN4 Products)

Synonyms: CB257 ELISA Kit, fbln4 ELISA Kit, fc56c09 ELISA Kit, id:ibd2923 ELISA Kit, sb:cb257 ELISA Kit, wu:fc56c09.x1 ELISA Kit, ARCL1B ELISA Kit, FBLN4 ELISA Kit, MBP1 ELISA Kit, UPH1 ELISA Kit, 0610011K11Rik ELISA Kit, Fbln4 ELISA Kit, FIBL-4 ELISA Kit, Fibulin-4 ELISA Kit, H411 ELISA Kit, EGF-containing fibulin-like extracellular matrix protein 2 ELISA Kit, EGF containing fibulin-like extracellular matrix protein 2b ELISA Kit, fibulin 4 ELISA Kit, EGF containing fibulin like extracellular matrix protein 2 ELISA Kit, epidermal growth factor-containing fibulin-like extracellular matrix protein 2 ELISA Kit, Efemp2 ELISA Kit, efemp2b ELISA Kit, fbln4 ELISA Kit, EFEMP2 ELISA Kit

Background:

Gene Name: Fibulin 4

Gene Aliases: EFEMP2, MBP1, UPH1, EGF-Containing Fibulin-Like Extracellular Matrix Protein 2

Gene ID: 30008

UniProt: O95967