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FGFBP3 CLIA Kit

FGFBP3 Reactivity: Human Chemiluminescent Sandwich ELISA 312.5 pg/mL - 20000 pg/mL Cell Lysate, Tissue Homogenate
Catalog No. ABIN5654303
  • Target See all FGFBP3 products
    FGFBP3 (Fibroblast Growth Factor Binding Protein 3 (FGFBP3))
    Reactivity
    • 1
    • 1
    Human
    Detection Method
    Chemiluminescent
    Method Type
    Sandwich ELISA
    Detection Range
    312.5 pg/mL - 20000 pg/mL
    Minimum Detection Limit
    312.5 pg/mL
    Application
    ELISA
    Sample Type
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor Binding Protein 3 (FGFBP3). No significant cross-reactivity or interference between Fibroblast Growth Factor Binding Protein 3 (FGFBP3) and analogues was observed.
    Sensitivity
    11.3 pg/mL
  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    2 - 3 h
    Plate
    Pre-coated
    Protocol
    The microplate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor Binding Protein 3 (FGFBP3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor Binding Protein 3 (FGFBP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibroblast Growth Factor Binding Protein 3 (FGFBP3) level in the sample or standard.,
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor Binding Protein 3 (FGFBP3) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor Binding Protein 3 (FGFBP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all FGFBP3 products
    FGFBP3 (Fibroblast Growth Factor Binding Protein 3 (FGFBP3))
    Alternative Name
    Fibroblast Growth Factor Binding Protein 3 (FGFBP3 Products)
    Synonyms
    C10orf13 CLIA Kit, FGF-BP3 CLIA Kit, 2610306H15Rik CLIA Kit, Fgf-bp3 CLIA Kit, RGD1560326 CLIA Kit, fibroblast growth factor binding protein 3 CLIA Kit, FGFBP3 CLIA Kit, Fgfbp3 CLIA Kit
    Background

    Gene Name: Fibroblast Growth Factor Binding Protein 3

    Gene Aliases: C10orf13

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