FGF3 CLIA Kit

Catalog No. ABIN5654257

Product Details FGF3 CLIA Kit

Target: FGF3 (Fibroblast Growth Factor 3 (FGF3))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 15.62 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.62 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 3 (FGF3). No significant cross-reactivity or interference between Fibroblast Growth Factor 3 (FGF3) and analogues was observed.

Sensitivity: 0.65 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 3 (FGF3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 3 (FGF3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibroblast Growth Factor 3 (FGF3) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 3 (FGF3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 3 (FGF3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: FGF3 (Fibroblast Growth Factor 3 (FGF3))

Alternative Name: Fibroblast Growth Factor 3 (FGF3 Products)

Synonyms: HBGF-3 CLIA Kit, INT2 CLIA Kit, Int2 CLIA Kit, Fgf-3 CLIA Kit, Int-2 CLIA Kit, Int-P CLIA Kit, INT-2 CLIA Kit, XFGF-3 CLIA Kit, fgf3-A CLIA Kit, xfgf3 CLIA Kit, FGF-3 CLIA Kit, id:ibd5006 CLIA Kit, fibroblast growth factor 3 CLIA Kit, fibroblast growth factor 3 L homeolog CLIA Kit, FGF3 CLIA Kit, Fgf3 CLIA Kit, fgf3.L CLIA Kit, fgf3 CLIA Kit

Background:

Gene Name: Fibroblast Growth Factor 3

Gene Aliases: HBGF3, INT2, Heparin-binding growth factor 3, Murine Mammary Tumor Virus Integration Site(v-int-2)Oncogene Homolog, INT-2 Proto-Oncogene Protein, Proto-oncogene Int-2

Gene ID: 2248

UniProt: P11487

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway