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FGF13 CLIA Kit

FGF13 Reactivity: Rat Chemiluminescent Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum
Catalog No. ABIN5654210
  • Target See all FGF13 CLIA Kits
    FGF13 (Fibroblast Growth Factor 13 (FGF13))
    Reactivity
    • 7
    • 3
    • 2
    Rat
    Detection Method
    Chemiluminescent
    Method Type
    Sandwich ELISA
    Detection Range
    15.62 pg/mL - 1000 pg/mL
    Minimum Detection Limit
    15.62 pg/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 13 (FGF13). No significant cross-reactivity or interference between Fibroblast Growth Factor 13 (FGF13) and analogues was observed.
    Sensitivity
    0.59 pg/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    2 - 3 h
    Plate
    Pre-coated
    Protocol
    The microplate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 13 (FGF13). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 13 (FGF13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibroblast Growth Factor 13 (FGF13) level in the sample or standard.,
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 13 (FGF13) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 13 (FGF13) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all FGF13 CLIA Kits
    FGF13 (Fibroblast Growth Factor 13 (FGF13))
    Alternative Name
    Fibroblast Growth Factor 13 (FGF13 Products)
    Synonyms
    FGF13 CLIA Kit, fgf2 CLIA Kit, fhf2 CLIA Kit, fgf13 CLIA Kit, FGF-13 CLIA Kit, xFGF13 CLIA Kit, FGF2 CLIA Kit, FHF-2 CLIA Kit, FHF2 CLIA Kit, Fhf2 CLIA Kit, zgc:101784 CLIA Kit, fibroblast growth factor 13 CLIA Kit, fibroblast growth factor 13 L homeolog CLIA Kit, fibroblast growth factor 13a CLIA Kit, FGF13 CLIA Kit, fgf13 CLIA Kit, fgf13.L CLIA Kit, Fgf13 CLIA Kit, fgf13a CLIA Kit
    Background

    Gene Name: Fibroblast Growth Factor 13

    Gene Aliases: FHF2, Fibroblast Growth Factor Homologous Factor 2

    Gene ID
    84488
    UniProt
    Q9ERW3
    Pathways
    Regulation of Cell Size
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