FGG ELISA Kit

Catalog No. ABIN5654156

Product Details FGG ELISA Kit

Target: FGG (Fibrinogen gamma Chain (FGG))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.81 ng/mL - 500 ng/mL

Minimum Detection Limit: 7.81 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Fibrinogen Gamma (FGg). No significant cross-reactivity or interference between Fibrinogen Gamma (FGg) and analogues was observed.

Sensitivity: 2.7 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibrinogen Gamma (FGg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibrinogen Gamma (FGg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibrinogen Gamma (FGg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibrinogen Gamma (FGg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibrinogen Gamma (FGg) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibrinogen Gamma (FGg) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for FGG

Target: FGG (Fibrinogen gamma Chain (FGG))

Alternative Name: Fibrinogen Gamma (FGG Products)

Synonyms: FXII ELISA Kit, HAF ELISA Kit, 3010002H13Rik ELISA Kit, AI256424 ELISA Kit, fibrinogen ELISA Kit, FGG ELISA Kit, LOC100220680 ELISA Kit, fb60h05 ELISA Kit, fb62e01 ELISA Kit, wu:fb60h05 ELISA Kit, wu:fb62e01 ELISA Kit, zgc:56023 ELISA Kit, fibrinogen gamma chain ELISA Kit, coagulation factor XII (Hageman factor) ELISA Kit, fibrinogen gamma chain L homeolog ELISA Kit, FGG ELISA Kit, F12 ELISA Kit, fgg ELISA Kit, Fgg ELISA Kit, fgg.L ELISA Kit, CpipJ_CPIJ006387 ELISA Kit, CpipJ_CPIJ010087 ELISA Kit

Background:

Gene Name: Fibrinogen Gamma

Gene Aliases: FG-G

Gene ID: 24367

UniProt: P02680