FABP7 ELISA Kit
-
- Target See all FABP7 ELISA Kits
- FABP7 (Fatty Acid Binding Protein 7, Brain (FABP7))
-
Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.469 ng/mL - 30 ng/mL
- Minimum Detection Limit
- 0.469 ng/mL
- Application
- ELISA
- Sample Type
- Plasma, Serum
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Fatty Acid Binding Protein 7, Brain (FABP7). No significant cross-reactivity or interference between Fatty Acid Binding Protein 7, Brain (FABP7) and analogues was observed.
- Sensitivity
- 0.18 ng/mL
- Featured
- Discover our best selling FABP7 ELISA Kit
- Top Product
- Discover our top product FABP7 ELISA Kit
-
-
- Comment
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 3 h
- Plate
- Pre-coated
- Protocol
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Binding Protein 7, Brain (FABP7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Binding Protein 7, Brain (FABP7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Binding Protein 7, Brain (FABP7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fatty Acid Binding Protein 7, Brain (FABP7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Assay Precision
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Binding Protein 7, Brain (FABP7) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Binding Protein 7, Brain (FABP7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
-
- Handling Advice
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
-
- Target See all FABP7 ELISA Kits
- FABP7 (Fatty Acid Binding Protein 7, Brain (FABP7))
- Alternative Name
- Fatty Acid Binding Protein 7, Brain (FABP7 Products)
- Synonyms
- B-FABP ELISA Kit, FABP7 ELISA Kit, D168 ELISA Kit, fabp7 ELISA Kit, wu:fb62f07 ELISA Kit, wu:fq20b08 ELISA Kit, BLBP ELISA Kit, FABPB ELISA Kit, MRG ELISA Kit, BFABP ELISA Kit, Blbp ELISA Kit, fatty acid binding protein 7 ELISA Kit, fatty acid binding protein 7, brain, a ELISA Kit, fatty acid-binding protein, brain ELISA Kit, fatty acid binding protein 7, brain ELISA Kit, fatty acid binding protein 7, brain L homeolog ELISA Kit, fatty acid binding protein 7, brain, b ELISA Kit, FABP7 ELISA Kit, Fabp7 ELISA Kit, fabp7a ELISA Kit, fabp7 ELISA Kit, fabp7.L ELISA Kit, fabp7b ELISA Kit
- Background
-
Gene Name: Fatty Acid Binding Protein 7, Brain
Gene Aliases: B-FABP, BLBP, FABPB, MRG, Brain Lipid Binding Protein, Mammary-derived growth inhibitor related
- Gene ID
- 2173
- UniProt
- O15540
-