FAAH ELISA Kit

Catalog No. ABIN5654062

Product Details FAAH ELISA Kit

Target: FAAH (Fatty Acid Amide Hydrolase (FAAH))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Fatty Acid Amide Hydrolase (FAAH). No significant cross-reactivity or interference between Fatty Acid Amide Hydrolase (FAAH) and analogues was observed.

Sensitivity: 0.58 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Amide Hydrolase (FAAH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Amide Hydrolase (FAAH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Amide Hydrolase (FAAH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fatty Acid Amide Hydrolase (FAAH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Amide Hydrolase (FAAH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Amide Hydrolase (FAAH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for FAAH

Target: FAAH (Fatty Acid Amide Hydrolase (FAAH))

Alternative Name: Fatty Acid Amide Hydrolase (FAAH Products)

Synonyms: AW412498 ELISA Kit, FAAH-1 ELISA Kit, FAAH ELISA Kit, faah ELISA Kit, faah-1 ELISA Kit, AtFAAH ELISA Kit, fatty acid amide hydrolase ELISA Kit, fatty acid amide hydrolase ELISA Kit, fatty-acid amide hydrolase 1 ELISA Kit, fatty-acid amide hydrolase ELISA Kit, fatty acid amide hydrolase, gene 1 S homeolog ELISA Kit, fatty acid amide hydrolase, gene 3 ELISA Kit, Faah ELISA Kit, FAAH ELISA Kit, LOC482510 ELISA Kit, faah ELISA Kit, NFIA_029220 ELISA Kit, NFIA_010900 ELISA Kit, ACLA_086700 ELISA Kit, BDBG_07484 ELISA Kit, faah.1.S ELISA Kit, Tsp_03856 ELISA Kit, faah.3 ELISA Kit

Background:

Gene Name: Fatty Acid Amide Hydrolase

Gene Aliases: FAAH-1, Anandamide amidohydrolase 1, Oleamide hydrolase 1

Pathways: Monocarboxylic Acid Catabolic Process