XPO5 ELISA Kit

Catalog No. ABIN5654021

Product Details XPO5 ELISA Kit

Target: XPO5 (Exportin 5 (XPO5))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Exportin 5 (XPO5). No significant cross-reactivity or interference between Exportin 5 (XPO5) and analogues was observed.

Sensitivity: 0.115 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Exportin 5 (XPO5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Exportin 5 (XPO5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Exportin 5 (XPO5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Exportin 5 (XPO5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Exportin 5 (XPO5) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Exportin 5 (XPO5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for XPO5

Target: XPO5 (Exportin 5 (XPO5))

Alternative Name: Exportin 5 (XPO5 Products)

Synonyms: exp5 ELISA Kit, CG12234 ELISA Kit, Dmel\\CG12234 ELISA Kit, Exp-5 ELISA Kit, Exp5 ELISA Kit, RanBP21 ELISA Kit, dmExp5 ELISA Kit, 2410004H11Rik ELISA Kit, 2700038C24Rik ELISA Kit, AI648907 ELISA Kit, AW549301 ELISA Kit, RanBp21 ELISA Kit, mKIAA1291 ELISA Kit, exportin-5 ELISA Kit, ranbp21 ELISA Kit, DDBDRAFT_0185980 ELISA Kit, DDBDRAFT_0237583 ELISA Kit, DDB_0185980 ELISA Kit, DDB_0237583 ELISA Kit, XPO5 ELISA Kit, exportin 5 ELISA Kit, CG12234 gene product from transcript CG12234-RB ELISA Kit, armadillo-like helical domain-containing protein ELISA Kit, XPO5 ELISA Kit, Ranbp21 ELISA Kit, Xpo5 ELISA Kit, xpo5 ELISA Kit

Background:

Gene Name: Exportin 5

Gene Aliases: RANBP21, Exp5, Ran-binding protein 21

Gene ID: 57510

UniProt: Q9HAV4

Pathways: Regulatory RNA Pathways, Protein targeting to Nucleus