XPO1 ELISA Kit

Catalog No. ABIN5654020

Product Details XPO1 ELISA Kit

Target: XPO1 (Exportin 1 (XPO1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Exportin 1 (XPO1). No significant cross-reactivity or interference between Exportin 1 (XPO1) and analogues was observed.

Sensitivity: 0.117 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Exportin 1 (XPO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Exportin 1 (XPO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Exportin 1 (XPO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Exportin 1 (XPO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Exportin 1 (XPO1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Exportin 1 (XPO1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for XPO1

Target: XPO1 (Exportin 1 (XPO1))

Alternative Name: Exportin 1 (XPO1 Products)

Synonyms: CG13387 ELISA Kit, CRM1 ELISA Kit, Crm1 ELISA Kit, DCRM1 ELISA Kit, Dmel\\CG13387 ELISA Kit, Emb ELISA Kit, XPO-1 ELISA Kit, XPO1 ELISA Kit, Xpo1 ELISA Kit, crm1 ELISA Kit, dCRM1 ELISA Kit, l(2)k16715 ELISA Kit, 18.m06600 ELISA Kit, DDBDRAFT_0183812 ELISA Kit, DDBDRAFT_0234066 ELISA Kit, DDB_0183812 ELISA Kit, DDB_0234066 ELISA Kit, exportin-1 ELISA Kit, LOC100220104 ELISA Kit, emb ELISA Kit, exp1 ELISA Kit, Xpo ELISA Kit, AA420417 ELISA Kit, Exp1 ELISA Kit, embargoed ELISA Kit, exportin 1 ELISA Kit, putative exportin 1 ELISA Kit, chromosome region maintenance protein 1 ELISA Kit, exportin-1 ELISA Kit, exportin 1 (CRM1 homolog, yeast) ELISA Kit, exportin 1 S homeolog ELISA Kit, emb ELISA Kit, cgd3_3060 ELISA Kit, Tc00.1047053511725.150 ELISA Kit, Tb11.01.5940 ELISA Kit, BBOV_II007220 ELISA Kit, LMJF_32_1100 ELISA Kit, xpo1 ELISA Kit, Xpo1 ELISA Kit, XPO1 ELISA Kit, LOC100165622 ELISA Kit, LOC100633509 ELISA Kit, xpo1.S ELISA Kit

Background:

Gene Name: Exportin 1

Gene Aliases: Exp1, CRM1 Homolog,Yeast, Chromosome region maintenance 1 protein homolog

Gene ID: 7514

UniProt: O14980

Pathways: M Phase