ERCC1 ELISA Kit

Catalog No. ABIN5654008

Product Details ERCC1 ELISA Kit

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1). No significant cross-reactivity or interference between Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1) and analogues was observed.

Sensitivity: 0.054 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ERCC1

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Alternative Name: Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1 Products)

Synonyms: COFS4 ELISA Kit, RAD10 ELISA Kit, UV20 ELISA Kit, Ercc-1 ELISA Kit, ercc1 ELISA Kit, T12H1.18 ELISA Kit, T12H1_18 ELISA Kit, UV REPAIR DEFICIENT 7 ELISA Kit, UVR7 ELISA Kit, ERCC excision repair 1, endonuclease non-catalytic subunit ELISA Kit, excision repair cross-complementing rodent repair deficiency, complementation group 1 ELISA Kit, DNA excision repair protein ERCC-1 ELISA Kit, nucleotide repair protein ELISA Kit, excision repair cross-complementation group 1 L homeolog ELISA Kit, excision repair cross-complementation group 1 ELISA Kit, ERCC1 ELISA Kit, Ercc1 ELISA Kit, CC1G_04546 ELISA Kit, CpipJ_CPIJ013903 ELISA Kit, PITG_13719 ELISA Kit, ercc1 ELISA Kit, ercc1.L ELISA Kit

Background:

Gene Name: Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1

Gene Aliases: UV20, DNA excision repair protein ERCC-1

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response