EIF4A2 ELISA Kit

Catalog No. ABIN5654006

Product Details EIF4A2 ELISA Kit

Target: EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Eukaryotic Translation Initiation Factor 4A2 (EIF4A2). No significant cross-reactivity or interference between Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) and analogues was observed.

Sensitivity: 0.059 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eukaryotic Translation Initiation Factor 4A2 (EIF4A2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eukaryotic Translation Initiation Factor 4A2 (EIF4A2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eukaryotic Translation Initiation Factor 4A2 (EIF4A2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for EIF4A2

Target: EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Alternative Name: Eukaryotic Translation Initiation Factor 4A2 (EIF4A2 Products)

Synonyms: BM-010 ELISA Kit, DDX2B ELISA Kit, EIF4A ELISA Kit, EIF4F ELISA Kit, eIF-4A-II ELISA Kit, eIF4A-II ELISA Kit, 4833432N07Rik ELISA Kit, Ddx2b ELISA Kit, Eif4 ELISA Kit, ddx2b ELISA Kit, eif4aii ELISA Kit, eif4f ELISA Kit, xeif4aii ELISA Kit, EIF4A2 ELISA Kit, EIF4AII ELISA Kit, wu:fd50g11 ELISA Kit, zgc:63783 ELISA Kit, eukaryotic translation initiation factor 4A2 ELISA Kit, eukaryotic translation initiation factor 4A2 L homeolog ELISA Kit, microRNA 1248 ELISA Kit, eukaryotic translation initiation factor 4A, isoform 2 ELISA Kit, Eif4a2 ELISA Kit, EIF4A2 ELISA Kit, eif4a2.L ELISA Kit, eif4a2 ELISA Kit, MIR1248 ELISA Kit

Background:

Gene Name: Eukaryotic Translation Initiation Factor 4A2

Gene Aliases: EIF4A, BM-010, DDX2B, EIF4F, ATP-dependent RNA helicase eIF4A-2