eEF1A1 ELISA Kit

Catalog No. ABIN5654003

Product Details eEF1A1 ELISA Kit

Target: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1). No significant cross-reactivity or interference between Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) and analogues was observed.

Sensitivity: 0.055 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for eEF1A1

Target: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Alternative Name: Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1A1 Products)

Synonyms: CCS-3 ELISA Kit, CCS3 ELISA Kit, EE1A1 ELISA Kit, EEF-1 ELISA Kit, EEF1A ELISA Kit, EF-Tu ELISA Kit, EF1A ELISA Kit, GRAF-1EF ELISA Kit, HNGC:16303 ELISA Kit, LENG7 ELISA Kit, PTI1 ELISA Kit, eEF1A-1 ELISA Kit, Eef1a2 ELISA Kit, Eef1a2l1 ELISA Kit, SI ELISA Kit, EEF1A2 ELISA Kit, EF1A1 ELISA Kit, eef1a1 ELISA Kit, wu:fj34g08 ELISA Kit, zgc:110335 ELISA Kit, EEF1A1 ELISA Kit, RABEFLA2 ELISA Kit, EFL1-alpha ELISA Kit, chunp6927 ELISA Kit, eef1a ELISA Kit, ef1a ELISA Kit, ik:tdsubc_2a3 ELISA Kit, ik:tdsubc_2b3 ELISA Kit, tdsubc_2a3 ELISA Kit, wu:fa91c07 ELISA Kit, wu:fa94b03 ELISA Kit, wu:fi13b09 ELISA Kit, xx:tdsubc_2a3 ELISA Kit, xx:tdsubc_2b3 ELISA Kit, EF-1A ELISA Kit, EF-1-ALPHA-S ELISA Kit, eef1a-s ELISA Kit, eef1as ELISA Kit, fj64c02 ELISA Kit, wu:fj64c02 ELISA Kit, zgc:73138 ELISA Kit, eukaryotic translation elongation factor 1 alpha 1 ELISA Kit, eukaryotic translation elongation factor 1 alpha 1b ELISA Kit, eukaryotic translation elongation factor 1 alpha 1, like 1 ELISA Kit, eukaryotic translation elongation factor 1 alpha 1 L homeolog ELISA Kit, elongation factor 1-alpha ELISA Kit, eukaryotic translation elongation factor 1 alpha 1a ELISA Kit, EEF1A1 ELISA Kit, Eef1a1 ELISA Kit, eef1a1b ELISA Kit, eef1a1l1 ELISA Kit, eef1a1.L ELISA Kit, tufA ELISA Kit, eef1a1a ELISA Kit

Background:

Gene Name: Eukaryotic Translation Elongation Factor 1 Alpha 1

Gene Aliases: CCS-3, CCS3, EEF-1, EEF1A, EF-Tu, EF1A, FLJ25721, GRAF-1EF, LENG7, PTI1, eEF1A-1, Leukocyte Receptor Cluster(LRC)Member 7, Elongation factor Tu

Gene ID: 1915

UniProt: P68104