Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Estrone CLIA Kit

Reactivity: Various Species Chemiluminescent Competition ELISA 23.44 pg/mL - 6000 pg/mL Plasma, Serum
Catalog No. ABIN5653996
  • Target See all Estrone CLIA Kits
    Estrone
    Reactivity
    • 5
    • 1
    Various Species
    Detection Method
    Chemiluminescent
    Method Type
    Competition ELISA
    Detection Range
    23.44 pg/mL - 6000 pg/mL
    Minimum Detection Limit
    23.44 pg/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Estrone (E1). No significant cross-reactivity or interference between Estrone (E1) and analogues was observed.
    Sensitivity
    7.96 pg/mL
    Featured
    Discover our best selling Estrone ELISA Kit
    Top Product
    Discover our top product Estrone ELISA Kit
  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    2 h
    Plate
    Pre-coated
    Protocol
    The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Estrone (E1). A competitive inhibition reaction is launched between biotin labeled Estrone (E1) and unlabeled Estrone (E1) (Standards or samples) with the pre-coated antibody specific to Estrone (E1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Estrone (E1) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Estrone (E1) level in the sample or standard.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Estrone (E1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Estrone (E1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all Estrone CLIA Kits
    Estrone
    Abstract
    Estrone Products
    Target Type
    Amino Acid
    Background

    Gene Name: Estrone

    Gene Aliases: Oestrone

You are here:
Support