EBI3 CLIA Kit

Catalog No. ABIN5653963

Product Details EBI3 CLIA Kit

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Reactivity: Mouse

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 156.25 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156.25 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Epstein Barr Virus Induced Protein 3 (EBI3). No significant cross-reactivity or interference between Epstein Barr Virus Induced Protein 3 (EBI3) and analogues was observed.

Sensitivity: 5.79 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Epstein Barr Virus Induced Protein 3 (EBI3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Epstein Barr Virus Induced Protein 3 (EBI3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Epstein Barr Virus Induced Protein 3 (EBI3) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epstein Barr Virus Induced Protein 3 (EBI3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Epstein Barr Virus Induced Protein 3 (EBI3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Alternative Name: Epstein Barr Virus Induced Protein 3 (IL-27b Products)

Synonyms: IL-27B CLIA Kit, IL27B CLIA Kit, EBI3 CLIA Kit, il-27rb CLIA Kit, si:dkey-97a13.4 CLIA Kit, EBI-3 CLIA Kit, IL-27 CLIA Kit, Epstein-Barr virus induced 3 CLIA Kit, Epstein-Barr virus induced gene 3 CLIA Kit, EBI3 CLIA Kit, ebi3 CLIA Kit, Ebi3 CLIA Kit

Target Type: Viral Protein

Background:

Gene Name: Epstein Barr Virus Induced Protein 3

Gene Aliases: IL27B, IL27-B, Interleukin 27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein

Gene ID: 50498

UniProt: O35228