EPH Receptor B3 ELISA Kit

Catalog No. ABIN5653919

Product Details EPH Receptor B3 ELISA Kit

Target: EPH Receptor B3 (EPHB3)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Ephrin Type B Receptor 3 (EPHB3). No significant cross-reactivity or interference between Ephrin Type B Receptor 3 (EPHB3) and analogues was observed.

Sensitivity: 0.051 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ephrin Type B Receptor 3 (EPHB3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ephrin Type B Receptor 3 (EPHB3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ephrin Type B Receptor 3 (EPHB3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ephrin Type B Receptor 3 (EPHB3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ephrin Type B Receptor 3 (EPHB3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ephrin Type B Receptor 3 (EPHB3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for EPH Receptor B3

Target: EPH Receptor B3 (EPHB3)

Alternative Name: Ephrin Type B Receptor 3 (EPHB3 Products)

Synonyms: tck ELISA Kit, MGC83457 ELISA Kit, EPHB3 ELISA Kit, ETK2 ELISA Kit, HEK2 ELISA Kit, TYRO6 ELISA Kit, AW456895 ELISA Kit, Cek10 ELISA Kit, Etk2 ELISA Kit, MDK5 ELISA Kit, Sek4 ELISA Kit, Tyro6 ELISA Kit, CEK10 ELISA Kit, EK10 ELISA Kit, ek3 ELISA Kit, ephb3 ELISA Kit, fc62d03 ELISA Kit, rtk3 ELISA Kit, wu:fc62d03 ELISA Kit, zek3 ELISA Kit, EPH receptor B3 ELISA Kit, EPH receptor B3 L homeolog ELISA Kit, Eph receptor B3 ELISA Kit, eph receptor B3a ELISA Kit, Eph receptor tyrosine kinase ELISA Kit, EPHB3 ELISA Kit, ephb3.L ELISA Kit, ephb3 ELISA Kit, Ephb3 ELISA Kit, ephb3a ELISA Kit

Background:

Gene Name: Ephrin Type B Receptor 3

Gene Aliases: ETK2, HEK2, TYRO6, EPH-like tyrosine kinase 2, Embryonic kinase 2, Tyrosine-protein kinase TYRO6

Gene ID: 13845

UniProt: P54754

Pathways: RTK Signaling