Ephrin A5 CLIA Kit

Catalog No. ABIN5653910

Product Details Ephrin A5 CLIA Kit

Target: Ephrin A5 (EFNA5)

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 156.25 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156.25 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Ephrin A5 (EFNA5). No significant cross-reactivity or interference between Ephrin A5 (EFNA5) and analogues was observed.

Sensitivity: 6.52 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Ephrin A5 (EFNA5). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ephrin A5 (EFNA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ephrin A5 (EFNA5) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ephrin A5 (EFNA5) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ephrin A5 (EFNA5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: Ephrin A5 (EFNA5)

Alternative Name: Ephrin A5 (EFNA5 Products)

Synonyms: EFNA5 CLIA Kit, af1 CLIA Kit, efl5 CLIA Kit, rags CLIA Kit, eplg7 CLIA Kit, lerk7 CLIA Kit, AL-1 CLIA Kit, AV158822 CLIA Kit, EFL-5 CLIA Kit, Ephrin-A5 CLIA Kit, Epl7 CLIA Kit, LERK-7 CLIA Kit, RAGS CLIA Kit, Lerk7 CLIA Kit, AF1 CLIA Kit, EFL5 CLIA Kit, EPLG7 CLIA Kit, GLC1M CLIA Kit, LERK7 CLIA Kit, ephrin A5 CLIA Kit, EFNA5 CLIA Kit, efna5 CLIA Kit, Efna5 CLIA Kit

Background:

Gene Name: Ephrin A5

Gene Aliases: AF1, EFL5, EPLG7, GLC1M, LERK7, RAGS, AL-1, EPH-related receptor tyrosine kinase ligand 7

Gene ID: 1946

UniProt: P52803

Pathways: RTK Signaling