EGLN1 ELISA Kit

Catalog No. ABIN5653787

Product Details EGLN1 ELISA Kit

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Egl Nine Homolog 1 (EGLN1). No significant cross-reactivity or interference between Egl Nine Homolog 1 (EGLN1) and analogues was observed.

Sensitivity: 13.5 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Egl Nine Homolog 1 (EGLN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Egl Nine Homolog 1 (EGLN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Egl Nine Homolog 1 (EGLN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Egl Nine Homolog 1 (EGLN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Egl Nine Homolog 1 (EGLN1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Egl Nine Homolog 1 (EGLN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for EGLN1

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Alternative Name: Egl Nine Homolog 1 (EGLN1 Products)

Synonyms: EGLN1 ELISA Kit, egln2 ELISA Kit, phd2 ELISA Kit, zgc:92314 ELISA Kit, AI503754 ELISA Kit, C1orf12 ELISA Kit, HIF-PH2 ELISA Kit, HPH-2 ELISA Kit, Hif-p4h-2 ELISA Kit, ORF13 ELISA Kit, Phd2 ELISA Kit, SM-20 ELISA Kit, PHD-2 ELISA Kit, PHD2 ELISA Kit, ECYT3 ELISA Kit, HIFPH2 ELISA Kit, HPH2 ELISA Kit, SM20 ELISA Kit, ZMYND6 ELISA Kit, egl nine homolog 1 (C. elegans) ELISA Kit, egl-9 family hypoxia inducible factor 1 ELISA Kit, egl-9 family hypoxia-inducible factor 1 S homeolog ELISA Kit, egl-9 family hypoxia-inducible factor 1b ELISA Kit, egl-9 family hypoxia-inducible factor 1 ELISA Kit, EGLN1 ELISA Kit, egln1.S ELISA Kit, egln1b ELISA Kit, Egln1 ELISA Kit

Background:

Gene Name: Egl Nine Homolog 1

Gene Aliases: ECYT3, HIFPH2, PHD2, C1orf12, SM20, ZMYND6, HIF Prolyl Hydroxylase 2, Prolyl hydroxylase domain-containing protein 2, Hypoxia-inducible factor prolyl hydroxylase 2

Pathways: cAMP Metabolic Process, Warburg Effect