ENPP1 ELISA Kit

Catalog No. ABIN5653779

Product Details ENPP1 ELISA Kit

Target: ENPP1 (Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1). No significant cross-reactivity or interference between Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) and analogues was observed.

Sensitivity: 0.063 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ENPP1

Target: ENPP1 (Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1))

Alternative Name: Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1 Products)

Synonyms: ENPP1 ELISA Kit, si:ch211-142e24.2 ELISA Kit, zgc:136238 ELISA Kit, ARHR2 ELISA Kit, M6S1 ELISA Kit, NPP1 ELISA Kit, NPPS ELISA Kit, PC-1 ELISA Kit, PCA1 ELISA Kit, PDNP1 ELISA Kit, 4833416E15Rik ELISA Kit, AI428932 ELISA Kit, C76301 ELISA Kit, CD203c ELISA Kit, E-NPP1 ELISA Kit, Ly-41 ELISA Kit, Npps ELISA Kit, Pca ELISA Kit, Pca-1 ELISA Kit, Pdnp1 ELISA Kit, ttw ELISA Kit, twy ELISA Kit, Pc1 ELISA Kit, ectonucleotide pyrophosphatase/phosphodiesterase 1 ELISA Kit, ectonucleotide pyrophosphatase/phosphodiesterase 1 L homeolog ELISA Kit, ENPP1 ELISA Kit, enpp1 ELISA Kit, enpp1.L ELISA Kit, Enpp1 ELISA Kit

Background:

Gene Name: Ectonucleotide Pyrophosphatase/Phosphodiesterase 1

Gene Aliases: M6S1, NPP1, NPPS, PC-1, PCA1, PDNP1, Membrane component chromosome 6 surface marker 1, Alkaline phosphodiesterase I, Plasma-cell membrane glycoprotein PC-1

Gene ID: 5167

UniProt: P22413

Pathways: Regulation of Carbohydrate Metabolic Process