E2F1 ELISA Kit

Catalog No. ABIN5653768

Product Details E2F1 ELISA Kit

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 g/mL - 10 g/mL

Minimum Detection Limit: 0.156 g/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of E2F Transcription Factor 1 (E2F1). No significant cross-reactivity or interference between E2F Transcription Factor 1 (E2F1) and analogues was observed.

Sensitivity: 0.054 g/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to E2F Transcription Factor 1 (E2F1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to E2F Transcription Factor 1 (E2F1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain E2F Transcription Factor 1 (E2F1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E2F Transcription Factor 1 (E2F1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level E2F Transcription Factor 1 (E2F1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level E2F Transcription Factor 1 (E2F1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F Transcription Factor 1 (E2F1 Products)

Synonyms: CG6376 ELISA Kit, DRTF1/E2F ELISA Kit, DmE2F-1 ELISA Kit, Dmel\\CG6376 ELISA Kit, Dp ELISA Kit, E(Sev-CycE)3A ELISA Kit, E(var)3-93E ELISA Kit, E(var)3-95E ELISA Kit, E(var)93E ELISA Kit, E2F ELISA Kit, E2F-1 ELISA Kit, E2F1 ELISA Kit, E2f1 ELISA Kit, Evar(3)164 ELISA Kit, dE2F ELISA Kit, dE2F1 ELISA Kit, dE2f ELISA Kit, dE2f1 ELISA Kit, de2f1 ELISA Kit, def21 ELISA Kit, drosE2F1 ELISA Kit, e2f ELISA Kit, e2f1 ELISA Kit, l(3)07172 ELISA Kit, l(3)j3B1 ELISA Kit, l(3)j3C2 ELISA Kit, l(3)rM729 ELISA Kit, RBAP1 ELISA Kit, RBBP3 ELISA Kit, RBP3 ELISA Kit, mKIAA4009 ELISA Kit, xE2F ELISA Kit, ATE2FB ELISA Kit, E2F TRANSCRIPTION FACTOR-1 E2F1 ELISA Kit, E2F transcription factor 1 ELISA Kit, E2FB ELISA Kit, E2FB TRANSCRIPTION FACTOR ELISA Kit, E2F transcription factor 1 ELISA Kit, E2F transcription factor 1 L homeolog ELISA Kit, E2f1 ELISA Kit, E2F1 ELISA Kit, e2f1.L ELISA Kit

Background:

Gene Name: E2F Transcription Factor 1

Gene Aliases: RBBP3, RBP3, RBAP-1, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, pRB-binding protein E2F-1

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy