DNAH11 ELISA Kit

Catalog No. ABIN5653756

Product Details DNAH11 ELISA Kit

Target: DNAH11 (Dynein, Axonemal, Heavy Chain 11 (DNAH11))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Dynein, Axonemal, Heavy Chain 11 (DNAH11). No significant cross-reactivity or interference between Dynein, Axonemal, Heavy Chain 11 (DNAH11) and analogues was observed.

Sensitivity: 0.127 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dynein, Axonemal, Heavy Chain 11 (DNAH11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dynein, Axonemal, Heavy Chain 11 (DNAH11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dynein, Axonemal, Heavy Chain 11 (DNAH11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dynein, Axonemal, Heavy Chain 11 (DNAH11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dynein, Axonemal, Heavy Chain 11 (DNAH11) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dynein, Axonemal, Heavy Chain 11 (DNAH11) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for DNAH11

Target: DNAH11 (Dynein, Axonemal, Heavy Chain 11 (DNAH11))

Alternative Name: Dynein, Axonemal, Heavy Chain 11 (DNAH11 Products)

Synonyms: CILD7 ELISA Kit, DNAHBL ELISA Kit, DNAHC11 ELISA Kit, DNHBL ELISA Kit, DPL11 ELISA Kit, Dnahc11 ELISA Kit, b2b1203Clo ELISA Kit, b2b1279Clo ELISA Kit, b2b1289Clo ELISA Kit, b2b1727Clo ELISA Kit, b2b598Clo ELISA Kit, iv ELISA Kit, lrd ELISA Kit, Bv1 ELISA Kit, dynein axonemal heavy chain 11 ELISA Kit, dynein, axonemal, heavy chain 11 ELISA Kit, dynein heavy chain 11, axonemal ELISA Kit, hypothetical protein LOC472305 ELISA Kit, dynein axonemal heavy chain 11 L homeolog ELISA Kit, DNAH11 ELISA Kit, Dnah11 ELISA Kit, LOC497208 ELISA Kit, LOC100497531 ELISA Kit, dnah11 ELISA Kit, dnah11.L ELISA Kit

Background:

Gene Name: Dynein, Axonemal, Heavy Chain 11

Gene Aliases: DNAHBL, DNAHC11, DNHBL, DPL11, Dynein,Heavy Chain Beta-Like, Axonemal beta dynein heavy chain 11, Ciliary dynein heavy chain 11

Gene ID: 8701

UniProt: Q96DT5