DYRK1A ELISA Kit

Catalog No. ABIN5653750

Product Details DYRK1A ELISA Kit

Target: DYRK1A (Dual-Specificity tyrosine-(Y)-phosphorylation Regulated Kinase 1A (DYRK1A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A). No significant cross-reactivity or interference between Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) and analogues was observed.

Sensitivity: 0.117 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for DYRK1A

Target: DYRK1A (Dual-Specificity tyrosine-(Y)-phosphorylation Regulated Kinase 1A (DYRK1A))

Alternative Name: Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A Products)

Synonyms: DYRK1A ELISA Kit, dyrk ELISA Kit, dyrk1 ELISA Kit, hp86 ELISA Kit, mnb ELISA Kit, mnbh ELISA Kit, DYRK ELISA Kit, DYRK1 ELISA Kit, HP86 ELISA Kit, MNB ELISA Kit, MNBH ELISA Kit, MRD7 ELISA Kit, 2310043O08Rik ELISA Kit, D16Ertd272e ELISA Kit, D16Ertd493e ELISA Kit, Dyrk ELISA Kit, ENSMUSG00000074897 ELISA Kit, Gm10783 ELISA Kit, Mnbh ELISA Kit, Mp86 ELISA Kit, mmb ELISA Kit, PSK47 ELISA Kit, dual specificity tyrosine phosphorylation regulated kinase 1A ELISA Kit, dual specificity tyrosine-(Y)-phosphorylation regulated kinase 1A ELISA Kit, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a ELISA Kit, dual specificity tyrosine-(Y)-phosphorylation regulated kinase 1A S homeolog ELISA Kit, DYRK1A ELISA Kit, dyrk1a ELISA Kit, Dyrk1a ELISA Kit, dyrk1a.S ELISA Kit

Background:

Gene Name: Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A

Gene Aliases: DYRK, DYRK1, HP86, MNB, MNBH, Dual specificity YAK1-related kinase, Protein kinase minibrain homolog

Gene ID: 1859

UniProt: Q13627

Pathways: Mitotic G1-G1/S Phases