DCLK1 ELISA Kit

Catalog No. ABIN5653736

Product Details DCLK1 ELISA Kit

Target: DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Doublecortin Like Kinase 1 (DCLK1). No significant cross-reactivity or interference between Doublecortin Like Kinase 1 (DCLK1) and analogues was observed.

Sensitivity: 0.235 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Doublecortin Like Kinase 1 (DCLK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Doublecortin Like Kinase 1 (DCLK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Doublecortin Like Kinase 1 (DCLK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Doublecortin Like Kinase 1 (DCLK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Doublecortin Like Kinase 1 (DCLK1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Doublecortin Like Kinase 1 (DCLK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for DCLK1

Target: DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))

Alternative Name: Doublecortin Like Kinase 1 (DCLK1 Products)

Synonyms: DCLK1 ELISA Kit, DCAMKL1 ELISA Kit, dcamkl1 ELISA Kit, dclk1 ELISA Kit, wu:fc51f11 ELISA Kit, zgc:153709 ELISA Kit, MGC145348 ELISA Kit, CL1 ELISA Kit, CLICK1 ELISA Kit, DCDC3A ELISA Kit, DCLK ELISA Kit, 1700113D08Rik ELISA Kit, 2810480F11Rik ELISA Kit, AI836758 ELISA Kit, Click-I ELISA Kit, Cpg16 ELISA Kit, Dcamkl1 ELISA Kit, Dcl ELISA Kit, Dclk ELISA Kit, mKIAA0369 ELISA Kit, Ania4 ELISA Kit, doublecortin like kinase 1 ELISA Kit, doublecortin-like kinase 1a ELISA Kit, serine/threonine-protein kinase DCLK1 ELISA Kit, doublecortin-like kinase 2 ELISA Kit, doublecortin-like kinase 1 ELISA Kit, DCLK1 ELISA Kit, dclk1a ELISA Kit, LOC587664 ELISA Kit, dclk2 ELISA Kit, LOC100539645 ELISA Kit, Dclk1 ELISA Kit

Background:

Gene Name: Doublecortin Like Kinase 1

Gene Aliases: DCDC3A, DCLK, DCAMKL1, Doublecortin And CaM Kinase-Like 1, Doublecortin domain-containing protein 3A, Doublecortin-like and CAM kinase-like 1

Gene ID: 9201

UniProt: O15075