DDOST ELISA Kit

Catalog No. ABIN5653717

Product Details DDOST ELISA Kit

Target: DDOST (Dolichyl-diphosphooligosaccharide--Protein Glycosyltransferase (DDOST))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST). No significant cross-reactivity or interference between Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST) and analogues was observed.

Sensitivity: 0.064 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for DDOST

Target: DDOST (Dolichyl-diphosphooligosaccharide--Protein Glycosyltransferase (DDOST))

Alternative Name: Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST Products)

Synonyms: AGER1 ELISA Kit, CDG1R ELISA Kit, OKSWcl45 ELISA Kit, OST ELISA Kit, OST48 ELISA Kit, WBP1 ELISA Kit, DDOST ELISA Kit, wu:fa11d01 ELISA Kit, zgc:66068 ELISA Kit, zgc:77478 ELISA Kit, Ddost ELISA Kit, CG9022 ELISA Kit, DmOST50 ELISA Kit, Dmel\\CG9022 ELISA Kit, DrOST ELISA Kit, OST48/WBP1 ELISA Kit, Ots48 ELISA Kit, anon-EST:Liang-1.11 ELISA Kit, clone 1.11 ELISA Kit, ost48 ELISA Kit, dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit ELISA Kit, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic) S homeolog ELISA Kit, dolichyl-diphosphooligosaccharide-protein glycosyltransferase ELISA Kit, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic) ELISA Kit, dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit ELISA Kit, dolichyl-di-phosphooligosaccharide-protein glycotransferase ELISA Kit, Oligosaccharyltransferase 48kD subunit ELISA Kit, DDOST ELISA Kit, ddost.S ELISA Kit, GTR26 ELISA Kit, ddost ELISA Kit, Ddost ELISA Kit, LOC5580056 ELISA Kit, PTRG_01946 ELISA Kit, Ost48 ELISA Kit

Background:

Gene Name: Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase

Gene Aliases: AGE-R1, OK/SW-cl.45, OST, OST48, WBP1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit, Oligosaccharyl transferase 48 kDa

Gene ID: 13200

UniProt: O54734

Pathways: Cell RedoxHomeostasis