DNMT1 ELISA Kit

Catalog No. ABIN5653706

Product Details DNMT1 ELISA Kit

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of DNA Methyltransferase 1 (DNMT1). No significant cross-reactivity or interference between DNA Methyltransferase 1 (DNMT1) and analogues was observed.

Sensitivity: 0.053 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNA Methyltransferase 1 (DNMT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DNA Methyltransferase 1 (DNMT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNA Methyltransferase 1 (DNMT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DNA Methyltransferase 1 (DNMT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DNA Methyltransferase 1 (DNMT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DNA Methyltransferase 1 (DNMT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Alternative Name: DNA Methyltransferase 1 (DNMT1 Products)

Synonyms: cb1075 ELISA Kit, cb983 ELISA Kit, dmnt1 ELISA Kit, fb05h01 ELISA Kit, mta ELISA Kit, wu:fb05h01 ELISA Kit, ADCADN ELISA Kit, AIM ELISA Kit, CXXC9 ELISA Kit, DNMT ELISA Kit, HSN1E ELISA Kit, MCMT ELISA Kit, DDM2 ELISA Kit, DECREASED DNA METHYLATION 2 ELISA Kit, DMT01 ELISA Kit, DMT1 ELISA Kit, DNA METHYLTRANSFERASE ELISA Kit, DNA METHYLTRANSFERASE 01 ELISA Kit, DNA METHYLTRANSFERASE 1 ELISA Kit, MET2 ELISA Kit, METHYLTRANSFERASE 2 ELISA Kit, METHYLTRANSFERASE I ELISA Kit, METI ELISA Kit, methyltransferase 1 ELISA Kit, Cxxc9 ELISA Kit, Dnmt ELISA Kit, Dnmt1o ELISA Kit, MTase ELISA Kit, Met-1 ELISA Kit, Met1 ELISA Kit, MommeD2 ELISA Kit, m.MmuI ELISA Kit, DNA methyltransferase 1 ELISA Kit, DNA (cytosine-5-)-methyltransferase 1 ELISA Kit, methyltransferase 1 ELISA Kit, DNA methyltransferase (cytosine-5) 1 ELISA Kit, DNMT1 ELISA Kit, dnmt1 ELISA Kit, Dnmt1 ELISA Kit, MET1 ELISA Kit

Background:

Gene Name: DNA Methyltransferase 1

Gene Aliases: CXXC9, DNMT, MCMT, Cytosine-5 DNA Methyltransferase 1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection