DLG4 ELISA Kit

Catalog No. ABIN5653698

Product Details DLG4 ELISA Kit

Target: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Discs, Large Homolog 4 (DLG4). No significant cross-reactivity or interference between Discs, Large Homolog 4 (DLG4) and analogues was observed.

Sensitivity: 0.056 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Discs, Large Homolog 4 (DLG4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Discs, Large Homolog 4 (DLG4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Discs, Large Homolog 4 (DLG4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Discs, Large Homolog 4 (DLG4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Discs, Large Homolog 4 (DLG4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Discs, Large Homolog 4 (DLG4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for DLG4

Target: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Alternative Name: Discs, Large Homolog 4 (DLG4 Products)

Synonyms: 11 ELISA Kit, CG1725 ELISA Kit, CG1730 ELISA Kit, CPD ELISA Kit, DLG ELISA Kit, DLG-A ELISA Kit, Discs-large ELISA Kit, Dlg ELISA Kit, Dlg-A ELISA Kit, Dlg1 ELISA Kit, DlgA ELISA Kit, Dmel\\CG1725 ELISA Kit, Drodlg ELISA Kit, PSD95 ELISA Kit, SAP97 ELISA Kit, anon-EST:Posey93 ELISA Kit, anon-WO03040301.258 ELISA Kit, anon-WO03040301.260 ELISA Kit, anon-WO03040301.268 ELISA Kit, d. lg.-1 ELISA Kit, dlg ELISA Kit, dlg-1 ELISA Kit, dlg-A ELISA Kit, dlgA ELISA Kit, dlgS97 ELISA Kit, l(1)10Bf ELISA Kit, l(1)G0276 ELISA Kit, l(1)G0342 ELISA Kit, l(1)G0456 ELISA Kit, l(1)G19 ELISA Kit, l(1)L11 ELISA Kit, l(1)bwn ELISA Kit, l(1)d.lg-1 ELISA Kit, l(1)d.lg.-1 ELISA Kit, l(1)discs large ELISA Kit, l(1)dlg ELISA Kit, l(1)dlg-1 ELISA Kit, l(1)dlg1 ELISA Kit, l(1)l.pr.-2 ELISA Kit, l(1)lpr-2 ELISA Kit, misb ELISA Kit, dlgh4 ELISA Kit, psd95 ELISA Kit, sap90 ELISA Kit, sap-90 ELISA Kit, LLGL1 ELISA Kit, Dlgh4 ELISA Kit, PSD-95 ELISA Kit, SAP90 ELISA Kit, SAP90A ELISA Kit, Sap90 ELISA Kit, SAP-90 ELISA Kit, DLG4 ELISA Kit, discs large 1 ELISA Kit, discs, large homolog 4 ELISA Kit, discs large MAGUK scaffold protein 4 ELISA Kit, discs, large homolog 4b (Drosophila) ELISA Kit, discs, large homolog 4 (Drosophila) ELISA Kit, dlg1 ELISA Kit, dlg4 ELISA Kit, DLG4 ELISA Kit, dlg4b ELISA Kit, Dlg4 ELISA Kit

Background:

Gene Name: Discs, Large Homolog 4

Gene Aliases: PSD95, SAP90, Postsynaptic density protein 95, Synapse-associated protein 90

Pathways: Regulation of Muscle Cell Differentiation, Synaptic Membrane, Skeletal Muscle Fiber Development, Asymmetric Protein Localization, Regulation of long-term Neuronal Synaptic Plasticity