DPP6 ELISA Kit

Catalog No. ABIN5653685

Product Details DPP6 ELISA Kit

Target: DPP6 (Dipeptidyl-Peptidase 6 (DPP6))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.12 ng/mL - 200 ng/mL

Minimum Detection Limit: 3.12 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Dipeptidyl Peptidase 6 (DPP6). No significant cross-reactivity or interference between Dipeptidyl Peptidase 6 (DPP6) and analogues was observed.

Sensitivity: 1.08 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dipeptidyl Peptidase 6 (DPP6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dipeptidyl Peptidase 6 (DPP6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dipeptidyl Peptidase 6 (DPP6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dipeptidyl Peptidase 6 (DPP6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dipeptidyl Peptidase 6 (DPP6) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dipeptidyl Peptidase 6 (DPP6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for DPP6

Target: DPP6 (Dipeptidyl-Peptidase 6 (DPP6))

Alternative Name: Dipeptidyl Peptidase 6 (DPP6 Products)

Synonyms: dppx ELISA Kit, DPPX ELISA Kit, VF2 ELISA Kit, DPP VI ELISA Kit, dpp6 ELISA Kit, si:ch211-198f16.1 ELISA Kit, B930011P16Rik ELISA Kit, D5Buc3 ELISA Kit, D5Buc4 ELISA Kit, D5Buc5 ELISA Kit, Dpp-6 ELISA Kit, Gm1377 ELISA Kit, In(5)6H-p ELISA Kit, Peplb ELISA Kit, Rw ELISA Kit, dipeptidyl peptidase like 6 ELISA Kit, dipeptidyl-peptidase 6 ELISA Kit, dipeptidyl-peptidase 6b ELISA Kit, dipeptidylpeptidase 6 ELISA Kit, DPP6 ELISA Kit, dpp6 ELISA Kit, BCAN_A2221 ELISA Kit, BSUIS_A2016 ELISA Kit, Bsph_2289 ELISA Kit, BMEA_A2239 ELISA Kit, dpp6b ELISA Kit, Dpp6 ELISA Kit

Background:

Gene Name: Dipeptidyl Peptidase 6

Gene Aliases: DPPX, Dipeptidylpeptidase 6, Dipeptidyl aminopeptidase-like protein 6, Dipeptidyl aminopeptidase-related protein, Dipeptidyl peptidase IV-like protein

Gene ID: 1804

UniProt: P42658