DGAT1 ELISA Kit

Catalog No. ABIN5653649

Product Details DGAT1 ELISA Kit

Target: DGAT1 (Diacylglycerol O-Acyltransferase 1 (DGAT1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1). No significant cross-reactivity or interference between Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) and analogues was observed.

Sensitivity: 13.9 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for DGAT1

Target: DGAT1 (Diacylglycerol O-Acyltransferase 1 (DGAT1))

Alternative Name: Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1 Products)

Synonyms: ARAT ELISA Kit, ARGP1 ELISA Kit, DGAT ELISA Kit, C75990 ELISA Kit, D15Ertd23e ELISA Kit, Dgat ELISA Kit, dgat1 ELISA Kit, wu:fd36g11 ELISA Kit, zgc:77691 ELISA Kit, DGAT1 ELISA Kit, ABX45 ELISA Kit, AS11 ELISA Kit, ATDGAT ELISA Kit, DIACYLGLYCEROL ACYLTRANSFERASE ELISA Kit, F27F23.24 ELISA Kit, RDS1 ELISA Kit, TRIACYLGLYCEROL BIOSYNTHESIS DEFECT 1 ELISA Kit, DDBDRAFT_0202877 ELISA Kit, DDBDRAFT_0304727 ELISA Kit, DDB_0202877 ELISA Kit, DDB_0304727 ELISA Kit, zgc:92327 ELISA Kit, diacylglycerol O-acyltransferase 1 ELISA Kit, diacylglycerol O-acyltransferase 1a ELISA Kit, diacylglycerol O-acyltransferase 1 L homeolog ELISA Kit, membrane bound O-acyl transferase (MBOAT) family protein ELISA Kit, diacylglycerol O-acyltransferase Dga1 ELISA Kit, diacylglycerol O-acyltransferase 1b ELISA Kit, DGAT1 ELISA Kit, Dgat1 ELISA Kit, dgat1a ELISA Kit, dgat1.L ELISA Kit, TAG1 ELISA Kit, dgat1 ELISA Kit, MGYG_02131 ELISA Kit, Tsp_02502 ELISA Kit, LOC100283803 ELISA Kit, dga1 ELISA Kit, dgat1b ELISA Kit

Background:

Gene Name: Diacylglycerol-O-Acyltransferase Homolog 1

Gene Aliases: ARGP1, DGAT, ARAT, ACAT-related gene product 1, Acyl-CoA retinol O-fatty-acyltransferase, Diglyceride acyltransferase

Gene ID: 8694

UniProt: O75907

Pathways: Hormone Transport