DMBT1 ELISA Kit

Catalog No. ABIN5653606

Product Details DMBT1 ELISA Kit

Target: DMBT1 (Deleted in Malignant Brain Tumors 1 (DMBT1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Lung Lavage Fluid, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Deleted In Malignant Brain Tumors 1 (DMBT1). No significant cross-reactivity or interference between Deleted In Malignant Brain Tumors 1 (DMBT1) and analogues was observed.

Sensitivity: 0.059 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Deleted In Malignant Brain Tumors 1 (DMBT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Deleted In Malignant Brain Tumors 1 (DMBT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Deleted In Malignant Brain Tumors 1 (DMBT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Deleted In Malignant Brain Tumors 1 (DMBT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Deleted In Malignant Brain Tumors 1 (DMBT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Deleted In Malignant Brain Tumors 1 (DMBT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for DMBT1

Target: DMBT1 (Deleted in Malignant Brain Tumors 1 (DMBT1))

Alternative Name: Deleted In Malignant Brain Tumors 1 (DMBT1 Products)

Synonyms: GP340 ELISA Kit, muclin ELISA Kit, CRP ELISA Kit, CRP-[a] ELISA Kit, CRP-[b] ELISA Kit, Crpd ELISA Kit, DBMT1 ELISA Kit, gp300 ELISA Kit, p80 ELISA Kit, DMBT1 ELISA Kit, gp340 ELISA Kit, deleted in malignant brain tumors 1 ELISA Kit, deleted in malignant brain tumors 1 protein ELISA Kit, gp340 ELISA Kit, deleted in malignant brain tumors 1 S homeolog ELISA Kit, DMBT1 ELISA Kit, Dmbt1 ELISA Kit, LOC100064515 ELISA Kit, dmbt1 ELISA Kit, ST201phi2-1p340 ELISA Kit, dmbt1.S ELISA Kit

Background:

Gene Name: Deleted In Malignant Brain Tumors 1

Gene Aliases: GP340, SAG, Muclin, Glycoprotein 340, Hensin, Salivary agglutinin, Surfactant pulmonary-associated D-binding protein

Pathways: Activation of Innate immune Response