DDO ELISA Kit

Catalog No. ABIN5653541

Product Details DDO ELISA Kit

Target: DDO (D-Aspartate Oxidase (DDO))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of D-Aspartate Oxidase (DDO). No significant cross-reactivity or interference between D-Aspartate Oxidase (DDO) and analogues was observed.

Sensitivity: 0.059 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to D-Aspartate Oxidase (DDO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to D-Aspartate Oxidase (DDO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain D-Aspartate Oxidase (DDO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of D-Aspartate Oxidase (DDO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level D-Aspartate Oxidase (DDO) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level D-Aspartate Oxidase (DDO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for DDO

Target: DDO (D-Aspartate Oxidase (DDO))

Alternative Name: D-Aspartate Oxidase (DDO Products)

Synonyms: DDO ELISA Kit, DDBDRAFT_0203041 ELISA Kit, DDBDRAFT_0238431 ELISA Kit, DDB_0203041 ELISA Kit, DDB_0238431 ELISA Kit, DDBDRAFT_0217305 ELISA Kit, DDBDRAFT_0238432 ELISA Kit, DDB_0217305 ELISA Kit, DDB_0238432 ELISA Kit, 5330420D20Rik ELISA Kit, 5730402C02Rik ELISA Kit, AI467244 ELISA Kit, DASOX ELISA Kit, DDO-1 ELISA Kit, DDO-2 ELISA Kit, D-aspartate oxidase ELISA Kit, D-amino-acid:oxygen oxidoreductase ELISA Kit, DDO ELISA Kit, IFG3 ELISA Kit, LACBIDRAFT_308911 ELISA Kit, PAS_chr3_0406 ELISA Kit, Amir_4180 ELISA Kit, Gbro_4657 ELISA Kit, ddo-2 ELISA Kit, ddo-1 ELISA Kit, Sros_8818 ELISA Kit, Kfla_2239 ELISA Kit, Gobs_3505 ELISA Kit, Snas_1168 ELISA Kit, ddo ELISA Kit, Ddo ELISA Kit

Background:

Gene Name: D-Aspartate Oxidase

Gene Aliases: DASOX, DDO-1, DDO-2

UniProt: Q99489