D Amino Acid Oxidase CLIA Kit

Catalog No. ABIN5653537

Product Details D Amino Acid Oxidase CLIA Kit

Target: D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 156.25 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156.25 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of D-Amino Acid Oxidase (DAO). No significant cross-reactivity or interference between D-Amino Acid Oxidase (DAO) and analogues was observed.

Sensitivity: 5.62 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to D-Amino Acid Oxidase (DAO). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to D-Amino Acid Oxidase (DAO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the D-Amino Acid Oxidase (DAO) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level D-Amino Acid Oxidase (DAO) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level D-Amino Acid Oxidase (DAO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Alternative Name: D-Amino Acid Oxidase (DAO Products)

Synonyms: DAAO CLIA Kit, DAMOX CLIA Kit, OXDA CLIA Kit, daao CLIA Kit, oxda CLIA Kit, damox CLIA Kit, DAO CLIA Kit, Dao1 CLIA Kit, DKFZp469N2232 CLIA Kit, AI987963 CLIA Kit, Dao-1 CLIA Kit, SC5F2A.23c CLIA Kit, wu:fb55b11 CLIA Kit, zgc:113922 CLIA Kit, ABP CLIA Kit, ABP1 CLIA Kit, DAO1 CLIA Kit, KAO CLIA Kit, MGC114783 CLIA Kit, Abp CLIA Kit, Abp1 CLIA Kit, D-amino acid oxidase CLIA Kit, D-amino-acid oxidase CLIA Kit, D-amino-acid oxidase, tandem duplicate 1 CLIA Kit, amine oxidase, copper containing 1 CLIA Kit, D-amino-acid oxidase L homeolog CLIA Kit, DAO CLIA Kit, Dao CLIA Kit, dao CLIA Kit, SCO6740 CLIA Kit, dao.1 CLIA Kit, AOC1 CLIA Kit, PTRG_02224 CLIA Kit, SJAG_02496 CLIA Kit, dao.L CLIA Kit, Aoc1 CLIA Kit

Background:

Gene Name: D-Amino Acid Oxidase

Gene Aliases: DAMOX